{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T11:14:38Z","timestamp":1781090078271,"version":"3.54.1"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Instituto de Investigaciones Sanitarias del Hospital Universitario La Paz (IdiPAZ)"},{"name":"Red de Investigacion en SIDA (AIDS Research Network) (RIS)","award":["RD07\/0006\/2007"],"award-info":[{"award-number":["RD07\/0006\/2007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>Currently, screening for anal high\u2010grade squamous intraepithelial lesions (anal HSIL) relies on anal cytology and high\u2010resolution anoscopy. Since this approach has limited sensitivity and specificity for detecting anal HSIL, there is increasing interest in the role of biomarkers for predicting anal HSIL. The aim of this study is to evaluate the diagnostic accuracy of HPV E6\/E7\u2010mRNA expression for the detection of anal HSIL in MSM infected with HIV, in comparison to DNA\u2010HR\u2010HPV and anal cytology. This cross\u2010sectional screening study included 101 MSM followed at the HIV\u2010unit of La Paz University Hospital. Intra\u2010anal swabs from patients participating in a screening program including cytology, high\u2010resolution anoscopy and histology were analyzed. HR\u2010HPV\u2010DNA detection was performed by means of the CLART\u00ae HPV2 assay (GENOMICA S.A.U., Madrid, Spain). E6\/E7\u2010mRNA detection of HR\u2010HPV\u2010types 16, 18, 31, 33, and 45 was performed using the NucliSENS\u2010EasyQ assay (BioM\u00e9rieux, Marcy \u013aEtoile, France). HR\u2010HPV DNA and HPVE6\/E7 mRNA were detected in 82% and 57% of the anal smears respectively. Anal cytology screening was abnormal in 70.3%. For the detection of HSIL sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 71.7%, 55.6%, 57.9%, and 69.8% for E6\/E7\u2010mRNA testing, respectively, compared to 100%, 31.5%, 55.4%, and 100% for HR\u2010HPV\u2010DNA testing and to 83%, 40.7%, 54.9%, 73.3% of cytology testing. In comparison with the other tests, HPVE6\/E7 mRNA testing yielded a lower clinical sensitivity but a higher clinical specificity and PPV for the detection of anal HSIL in MSM infected with HIV. <jats:bold><jats:italic>J. Med. Virol. 87:1397\u20131403, 2015<\/jats:italic>.<\/jats:bold> \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24188","type":"journal-article","created":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T21:11:03Z","timestamp":1430514663000},"page":"1397-1403","source":"Crossref","is-referenced-by-count":27,"title":["Human papillomavirus mRNA testing for the detection of anal high\u2010grade squamous intraepithelial lesions in men who have sex with men infected with HIV"],"prefix":"10.1002","volume":"87","author":[{"given":"Elena","family":"Sendagorta","sequence":"first","affiliation":[{"name":"Department of Dermatology Hospital Universitario La Paz\u2010IdiPaz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria P.","family":"Romero","sequence":"additional","affiliation":[{"name":"Department of Microbiology Hospital Universitario La Paz\u2010IdiPaz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose I.","family":"Bernardino","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Hospital Universitario La Paz\u2010IdiPaz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J.","family":"Beato","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital Universitario La Paz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Alvarez\u2010Gallego","sequence":"additional","affiliation":[{"name":"Department of Coloproctology Hospital Universitario La Paz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Herranz","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hospital Universitario La Paz\u2010IdiPaz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"28","article-title":"EUROGIN 2011 roadmap on prevention and treatment of HPV\u2010related disease","volume":"24","author":"Arbyn M","year":"2012","journal-title":"Int J Cancer"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/DCR.0b013e31819793d9"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00126334-200412150-00004"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2010.11.013"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/505219"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199810000-00010"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(09)70009-5"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.297.8.813"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/COH.0b013e32831a6fe0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e328334fe5a"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20364"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.09712.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.27989"},{"key":"e_1_2_6_15_1","first-page":"1","article-title":"Anal human papillomavirus infection and associated neoplastic lesions in men who have sex with men: A systematic review and meta\u2010analysis","volume":"22","author":"Machalek DA","year":"2012","journal-title":"Lancet Oncol"},{"key":"e_1_2_6_16_1","unstructured":"Management of STIs in HIV\u2010Infected Patients. 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