{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T18:50:57Z","timestamp":1780599057163,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T00:00:00Z","timestamp":1426464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Brazilian Federal Agency and Evaluation of Graduate Education (CAPES)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>Polyomavirus BK (BKPyV) T\u2010antigens (large and small tumor antigens, or Lt\u2010ag and st\u2010ag, respectively), control key aspects of viral replication and are able to regulate cell cycle, promoting cell proliferation. However, the structural effects of genetic mutations on T\u2010antigens are poorly investigated. In this study, 214 sequences of T\u2010antigens from individuals with different BKPyV infections (16 renal transplant with nephropathy; 78 asymptomatic renal transplant; 24 hematopoietic stem cell transplant with hemorrhagic cystitis; 96 healthy non\u2010transplant), were analyzed from the genetic and structural standpoints. We found a high concentration of non\u2010synonymous mutations at inter\u2010domains and hexamerization regions of both proteins, being five of them under positive selection in the Lt\u2010ag but none in the st\u2010ag. The in silico analysis indicated that two mutations, located at positions 164 in the st\u2010ag and 592 in the Lt\u2010ag, would significantly affect the interaction with PP2A and p53 cell targets, respectively, although they were not associated to a specific clinical status. No mutations were detected on the J\u2010domains or at the ATPase motif. In sum, the profile of the mutations found seem not to be associated to increased morbidity. This is the first work to analyze structural modifications on T\u2010antigens in different BKPyV infections, and managed to map conserved and variable regions of the T\u2010antigens, which will be helpful for the study of new antiviral drugs. <jats:bold><jats:italic>J. Med. Virol. 87:1418\u20131426, 2015<\/jats:italic>.<\/jats:bold> \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24192","type":"journal-article","created":{"date-parts":[[2015,3,17]],"date-time":"2015-03-17T01:00:02Z","timestamp":1426554002000},"page":"1418-1426","source":"Crossref","is-referenced-by-count":4,"title":["Genetic and structural analysis of polyomavirus BK T\u2010antigens reveal a higher density of mutations at inter\u2010domain and hexamerization regions, regardless the status of infection"],"prefix":"10.1002","volume":"87","author":[{"given":"Kethellin M.","family":"Cardoso","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology Universidade Federal Fluminense Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria C.","family":"Diaz","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biotechnology Universidade Federal do Rio do Janeiro Rio de Janeiro 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