{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T07:41:37Z","timestamp":1696405297509},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:label \/><jats:p>We compared multiplex E6 messenger ribonucleic acid (mRNA) tests using real\u2010time quantitative reverse transcriptase polymerase chain reactions (PCR) with human papillomavirus (HPV) DNA subtypes using a MY11\/GP6+ PCR\u2010based reverse\u2010blot assay to identify cervical intraepithelial neoplasias of grade 2 or worse (CIN2+). In total, 684 women were studied, of whom 377 (55%) were diagnosed with CIN2+ histologically. The specificity of HPV mRNA to predict histological CIN2+ was higher than that of HPV DNA (81.3% vs. 44.2%). The odds ratios (ORs) to predict histological CIN2+ in women with positive for type 16, 18, 31, and 45 E6 mRNA or by HPV DNA detection were 7.1 (95% confidence interval [CI] 3.9\u201313.1) and 2.5 (95%CI 1.9\u20133.5), respectively, compared to those with negative for E6 mRNA or HPV DNA. The OR to predict histological CIN2+ in women with a cytological grade &lt;CIN2+ and positive for type 16, 18, 31, and 45 E6 mRNA was 9.7 (95%CI 3.2\u201329.2), compared to those with a cytological grade &lt;CIN2+, and negative for E6 mRNA (OR\u2009=\u20091), those with a cytological grade CIN2+, and negative for mRNA (OR\u2009=\u20096.9, 95%CI 4.4\u201310.8), and those with a cytological grade CIN2+ and positive for mRNA (OR\u2009=\u200928.0, 95%CI 9.8\u201379.6). As a HPV DNA positive triage, the OR to predict histological CIN2+ in women with a cytological grade &lt;CIN2+ and positive for mRNA was higher than those with negative for mRNA (OR:12.8 [95%CI 3.6\u20135.4] vs. OR:1.6 [95%CI 0.9\u20132.9]). In conclusion, multiplex HPV E6 mRNA detection can be used as a triage for women with cytological grade &lt;CIN2+. <jats:bold><jats:italic>J. Med. Virol. 87:1404\u20131412, 2015<\/jats:italic>.<\/jats:bold> \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24194","type":"journal-article","created":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T21:10:57Z","timestamp":1430514657000},"page":"1404-1412","source":"Crossref","is-referenced-by-count":0,"title":["Clinical performance of multiplex high\u2010risk e6 mrna expression in comparison with hpv dna subtypes for the identification of women at risk of cervical cancer"],"prefix":"10.1002","volume":"87","author":[{"given":"Chih\u2010Ming","family":"Ho","sequence":"first","affiliation":[{"name":"Gynecologic Cancer Center Department of Obstetrics and Gynecology Cathay General Hospital Taipei Taiwan"},{"name":"School of Medicine Fu Jen Catholic University Hsinchuang New Taipei City Taiwan"},{"name":"School of Medicine Taipei Medical University Taipei 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