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The present study aims to assess whether oxidative DNA damage is correlated with the grade of HPV\u2010related lesions. Moreover, we evaluated clinical data and unhealthy lifestyles to verify their possible influence on the genesis of oxidative DNA damage in cervical cells. We quantified the amount of 8\u2010Oxo\u20102\u2032\u2010deoxyguanosine in DNA as a biomarker of oxidative damage in women with and without HPV infection. We also correlated oxidative damage with different stages of cervical lesions and available clinical data (e.g., HPV genotypes). To identify HPV infections, in which proteins with a transforming potential are produced, we performed a qualitative detection of HPV E6\/E7 mRNA. Our results showed greater oxidative damage in HPV\u2010related dysplastic cervical lesions compared to samples with normal cytology, especially in women with high\u2010grade squamous intraepithelial lesions. The latter showed a closed link with high\u2010risk HPV genotypes. Reactive oxygen species can induce DNA double\u2010strand breaks in both the host DNA and in the circular viral episome; this could facilitate the integration of the virus, promoting HPV carcinogenesis. Therefore, in HPV\u2010infected women, it could be useful to reduce additional resources of reactive oxygen\/nitrogen species (RONS) with a healthy lifestyle. <jats:bold><jats:italic>J. Med. 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