{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:45:20Z","timestamp":1765485920348},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,10,5]],"date-time":"2015-10-05T00:00:00Z","timestamp":1444003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:sec><jats:label \/><jats:p>To compare the presence of human bocavirus (HBoV) in nasopharyngeal aspirates (NPA) versus broncho\u2010alveolar lavage fluids (BAL) in children with lower respiratory tract infections (LRTIs), as revealed by real\u2010time PCR, in order to confirm the diagnostic validity of NPA samples. A retrospective 5\u2010year study was performed from 2009 to 2014 in 1,194 patients under the age of 17 years (mean age of 3 years) that were diagnosed with LRTIs and from whom both NPA and BAL were obtained. Clinical and demographic data were recorded, and NPA and BAL samples were analyzed for HBoV\u2010positivity by real\u2010time PCR. Of the 1,194 patients enrolled, 65 (5.4%) patients had HBoV detected from NPA, and 61 (5.1%) had HBoV detected from BAL. For HBoV, there was a significant association between the NPA and BAL samples (<jats:italic>P\u2009<\/jats:italic>&lt; 0.001), but the diagnostic validity was relatively low (kappa\u2009=\u20090.414). When real\u2010time PCR\u2010positivity for HBoV in BAL was used as a reference for diagnosis, NPA had a good specificity and better positive predictive validity in male patients or those younger than 3 years of age. NPA has a similar yield and a good specificity for diagnosis of LRTIs with HBoV compared to BAL. The best diagnostic validity for NPA was detected in male patients or those younger than 3 years old. <jats:bold><jats:italic>J. Med. Virol. 88:211\u2013215, 2016<\/jats:italic>.<\/jats:bold> \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24338","type":"journal-article","created":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T13:42:54Z","timestamp":1438695774000},"page":"211-215","source":"Crossref","is-referenced-by-count":9,"title":["Detection of human bocavirus in nasopharyngeal aspirates versus in broncho\u2010alveolar lavage fluids in children with lower respiratory tract infections"],"prefix":"10.1002","volume":"88","author":[{"given":"Ling","family":"Li","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine Children's Hospital of Soochow University Suzhou China"},{"name":"Department of Pediatrics Suzhou Municipal Hospital Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Zhu","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Modern Silk, College of Textile and Clothing 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