{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:44:04Z","timestamp":1789811044953,"version":"4.0.1"},"reference-count":56,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,10,19]],"date-time":"2015-10-19T00:00:00Z","timestamp":1445212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fondecyt","award":["1080396"],"award-info":[{"award-number":["1080396"]}]},{"name":"Comision Nacional de Investigacion Cientifica y Tecnologica (Conicyt) from Chile (Support Scholarship for Doctoral Thesis)","award":["24090150"],"award-info":[{"award-number":["24090150"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>Human T\u2010lymphotropic virus\u2010type 1 (HTLV\u20101) is the etiologic agent of the neurologic disease HTLV\u20101\u2010associated myelopathy\/tropical spastic paraparesis (HAM\/TSP). Tax viral protein plays a critical role in viral pathogenesis. Previous studies suggested that extracellular Tax might involve cytokine\u2010like extracellular effects. We evaluated Tax secretion in 18 h\u2010ex vivo peripheral blood mononuclear cells (PBMCs) cultures from 15 HAM\/TSP patients and 15 asymptomatic carriers. Futhermore, Tax plasma level was evaluated from other 12 HAM\/TSP patients and 10 asymptomatic carriers. Proviral load and mRNA encoding Tax were quantified by PCR and real\u2010time RT\u2010PCR, respectively. Intracellular Tax in CD4(+)CD25(+) cells occurred in 100% and 86.7% of HAM\/TSP patients and asymptomatic carriers, respectively. Percentage of CD4(+)CD25(+) Tax+, proviral load and mRNA encoding Tax were significantly higher in HAM\/TSP patients. Western blot analyses showed higher secretion levels of ubiquitinated Tax in HAM\/TSP patients than in asymptomatic carriers. In HTLV\u20101\u2010infected subjects, Western blot of plasma Tax showed higher levels in HAM\/TSP patients than in asymptomatic carriers, whereas no Tax was found in non\u2010infected subjects. Immunoprecipitated plasma Tax resolved on SDS\u2013PAGE gave two major bands of 57 and 48\u2009kDa allowing identification of Tax and Ubiquitin peptides by mass spectrometry. Relative percentage of either CD4(+)CD25(+) Tax+ cells, or Tax protein released from PBMCs, or plasma Tax, correlates neither with tax mRNA nor with proviral load. This fact could be explained by a complex regulation of Tax expression. Tax secreted from PBMCs or present in plasma could potentially become a biomarker to distinguish between HAM\/TSP patients and asymptomatic carriers. <jats:bold><jats:italic>J. Med. Virol. 88:521\u2013531, 2016<\/jats:italic>.<\/jats:bold> \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24342","type":"journal-article","created":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T13:46:55Z","timestamp":1438696015000},"page":"521-531","source":"Crossref","is-referenced-by-count":12,"title":["Tax secretion from peripheral blood mononuclear cells and tax detection in plasma of patients with human T\u2010lymphotropic virus\u2010type 1\u2010associated myelopathy\/tropical spastic paraparesis and asymptomatic carriers"],"prefix":"10.1002","volume":"88","author":[{"given":"Fernando","family":"Medina","sequence":"first","affiliation":[{"name":"Faculty of Chemical and Pharmaceutical Sciences Department of Biochemistry and Molecular Biology University of Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n","family":"Quintremil","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Pharmaceutical Sciences Department of Biochemistry and Molecular Biology University of Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Alberti","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Pharmaceutical Sciences Department of Biochemistry and Molecular Biology University of Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabi\u00e1n","family":"Godoy","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Pharmaceutical Sciences Department of Biochemistry and Molecular Biology University of Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda E.","family":"Pando","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Pharmaceutical Sciences Department of Biochemistry and Molecular Biology University of Chile Santiago 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