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Current anti\u2010HBV medications have a number of drawbacks, such as adverse effects and drug resistance; thus, novel potential anti\u2010HBV reagents are needed. Selenium (Se) has been shown to be involved in both human immunodeficiency virus and hepatitis C virus infections, but its role in HBV infection remains unclear. To address this, sodium selenite (Na<jats:sub>2<\/jats:sub>SeO<jats:sub>3<\/jats:sub>) was applied to three HBV cell models: HepG2.2.15 cells, and HuH\u20107 cells transfected with either 1.1 or 1.3\u00d7 HBV plasmids. Cytotoxicity of Na<jats:sub>2<\/jats:sub>SeO<jats:sub>3<\/jats:sub> was examined by Cell Counting Kit\u20108. Levels of viral antigen expression, transcripts, and encapsidated viral DNA were measured by enzyme\u2010linked immunosorbent assay, northern blot, and Southern blot, respectively. There was no obvious cytotoxicity in either HepG2.2.15 or HuH\u20107 cells with &lt;2.5\u2009\u00b5M Na<jats:sub>2<\/jats:sub>SeO<jats:sub>3<\/jats:sub>. Below this concentration, Na<jats:sub>2<\/jats:sub>SeO<jats:sub>3<\/jats:sub> suppressed HBsAg and HBeAg production, HBV transcript level, and amount of genomic DNA in all three tested models, and suppression level was enhanced in line with increases in Na<jats:sub>2<\/jats:sub>SeO<jats:sub>3<\/jats:sub> concentration or treatment time. Moreover, the inhibitory effect of Na<jats:sub>2<\/jats:sub>SeO<jats:sub>3<\/jats:sub> on HBV replication can be further enhanced by combined treatment with lamivudine, entecavir, or adefovir. Thus, the present study clearly proves that Na<jats:sub>2<\/jats:sub>SeO<jats:sub>3<\/jats:sub> suppresses HBV protein expression, transcription, and genome replication in hepatoma cell models in a dose\u2010 and time\u2010dependent manner. <jats:bold><jats:italic>J. Med. Virol. 88:653\u2013663, 2016<\/jats:italic>.<\/jats:bold> \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24366","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T15:32:57Z","timestamp":1441207977000},"page":"653-663","source":"Crossref","is-referenced-by-count":44,"title":["Sodium selenite suppresses hepatitis B virus transcription and replication in human hepatoma cell lines"],"prefix":"10.1002","volume":"88","author":[{"given":"Zhikui","family":"Cheng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoguang","family":"Zhi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 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