{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T18:25:57Z","timestamp":1750962357856},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,9,23]],"date-time":"2015-09-23T00:00:00Z","timestamp":1442966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec><jats:label \/><jats:p>B19V infection is common during childhood. It is self\u2010limited in healthy individuals, but is often associated with transient aplastic crisis in children with sickle cell disease. The aim of this study was to estimate the prevalence and incidence of B19V infection in children with sickle cell disease screened by the Newborn Screening Program of Minas Gerais, Brazil, and followed\u2010up at Funda\u00e7\u00e3o Hemominas. Serum or plasma samples from 278 patients were tested for anti\u2010B19V IgG and IgM using commercial ELISA and for viral DNA using in\u2010house real\u2010time PCR assays; 127 negative\u2010children were retested about 1 year later. The median age of children at first testing was 5.9 years (0.8\u201312.3). The estimated prevalence of B19V was 29.5 % (95%CI 24.1\u201334.9 %). The incidence of B19V in those 127 negative\u2010children was 18.2 cases\/100 patient\u2010years. All DNA\u2010positive samples were identified as genotype 1, except one sample, in which both genotypes 1 and 3 were identified. It was observed that the higher the child's age, the higher the probability of B19V infection. The analysis of clinical and hematological data showed a significant association of B19V infection with transient aplastic crisis and acute splenic sequestration, higher frequency of transfusions, and higher rate of hospitalization, but not with acute chest syndrome or stroke. These results emphasize the impact of B19V infection on the course of sickle cell disease. Strategies to prevent and monitor B19V infection in children with sickle cell disease should be considered to diminish its morbidity in this susceptible population. <jats:bold><jats:italic>J. Med. Virol. 88:588\u2013595, 2016<\/jats:italic>.<\/jats:bold> \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24378","type":"journal-article","created":{"date-parts":[[2015,9,15]],"date-time":"2015-09-15T11:52:51Z","timestamp":1442317971000},"page":"588-595","source":"Crossref","is-referenced-by-count":11,"title":["Prevalence and incidence of erythrovirus B19 infection in children with sickle cell disease: The impact of viral infection in acute clinical events"],"prefix":"10.1002","volume":"88","author":[{"given":"Marina","family":"dos Santos Brito Silva Furtado","sequence":"first","affiliation":[{"name":"Servi\u00e7o de Pesquisa Funda\u00e7\u00e3o Hemominas Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos","family":"Borato Viana","sequence":"additional","affiliation":[{"name":"Departamento de Pediatriae Nupad, Faculdade de Medicina Universidade Federal de Minas Gerais Belo Horizonte 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