{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T18:32:41Z","timestamp":1771007561251,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T00:00:00Z","timestamp":1446768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:label\/><jats:p>To compare the diagnostic performance for HPV infection using three laboratorial techniques. Ninty\u2010five cervicovaginal samples were randomly selected; each was tested for HPV DNA and genotypes using 3 methods in parallel: Multiplex\u2010PCR, the Nested PCR followed by Sanger sequencing, and the Next_Gen Sequencing (NGS) with two assays (NGS\u2010A1, NGS\u2010A2). The study was approved by the Brazilian National IRB (CONEP protocol 16,800). The prevalence of HPV by the NGS assays was higher than that using the Multiplex\u2010PCR (64.2% vs. 45.2%, respectively; <jats:italic>P<\/jats:italic>\u2009=\u20090.001) and the Nested\u2010PCR (64.2% vs. 49.5 %, respectively; <jats:italic>P<\/jats:italic>\u2009=\u20090.003). NGS also showed better performance in detecting high\u2010risk HPV (HR\u2010HPV) and HPV16. There was a weak interobservers agreement between the results of Multiplex\u2010PCR and Nested\u2010PCR in relation to NGS for the diagnosis of HPV infection, and a moderate correlation for HR\u2010HPV detection. Both NGS assays showed a strong correlation for detection of HPVs (k\u2009=\u20090.86), HR\u2010HPVs (k\u2009=\u20090.91), HPV16 (k\u2009=\u20090.92) and HPV18 (k\u2009=\u20090.91). NGS is more sensitive than the traditional Sanger sequencing and the Multiplex PCR to genotype HPVs, with promising ability to detect multiple infections, and may have the potential to establish an alternative method for the diagnosis and genotyping of HPV. <jats:bold><jats:italic>J. Med. Virol. 88:888\u2013894, 2016<\/jats:italic>.<\/jats:bold> \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24413","type":"journal-article","created":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T23:08:45Z","timestamp":1445641725000},"page":"888-894","source":"Crossref","is-referenced-by-count":22,"title":["Comparison of three human papillomavirus DNA detection methods: Next generation sequencing, multiplex\u2010PCR and nested\u2010PCR followed by Sanger based sequencing"],"prefix":"10.1002","volume":"88","author":[{"given":"Allex Jardim","family":"da Fonseca","sequence":"first","affiliation":[{"name":"Tropical Medicine Foundation Dr. Heitor Vieira Dourado University of State of Amazonas Manaus Brazil"},{"name":"Center of Infectious Diseases Research Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata Silva","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"Tropical Medicine Foundation Dr. Heitor Vieira Dourado University of State of Amazonas Manaus Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelica Espinosa","family":"Miranda","sequence":"additional","affiliation":[{"name":"Center of Infectious Diseases Research Federal University of Esp\u00edrito Santo Vit\u00f3ria Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Carlos de Lima","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Tropical Medicine Foundation Dr. Heitor Vieira Dourado University of State of Amazonas Manaus Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zigui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology Epidemiology &amp; Population Health, and Obstetrics, Gynecology &amp; Women's Health Albert Einstein College of Medicine New York"},{"name":"Department of Microbiology Faculty of Medicine The Chinese University of Hong Kong Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,11,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1991.03460040048027"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/657321"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2013-6_1"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(05)70086-3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-76-4-1057"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70230-8"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2013.04.023"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.03.033"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq461"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr381"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0133635"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.08.007"},{"key":"e_1_2_6_14_1","first-page":"121","article-title":"Polymerase chain reaction\u2010based methods for the detection of human papillomavirus DNA","volume":"119","author":"Gravitt PE","year":"1992","journal-title":"IARC Sci Pub"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000355952"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(00)00194-4"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr507"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr026"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1156"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-284"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040425"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.10.024"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2014.00150"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP9P2KJSXEKCJB"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.24413","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.24413","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T13:49:05Z","timestamp":1696600145000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.24413"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,6]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1002\/jmv.24413"],"URL":"https:\/\/doi.org\/10.1002\/jmv.24413","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,6]]}}}