{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T04:14:03Z","timestamp":1748751243936,"version":"3.41.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,11,9]],"date-time":"2016-11-09T00:00:00Z","timestamp":1478649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003323","name":"Agence Nationale de Recherches sur le Sida et les Hepatites Virales","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003323","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>The aim of this pilot study was to analyze the Hepatitis C Virus (HCV) genotypes circulating in Senegal among Drug User (DUs), using Dried Blood Spots (DBS) as RNA source for molecular assays. Heroin and\/or cocaine users (n\u2009=\u2009506) were recruited in Dakar from April to July 2011, using a Respondent Driven Sampling (RDS) method. DBS preparation consisted of five drops of whole blood from finger applied to a Whatman paper card. HCV infection was screened by the detection of anti\u2010HCV antibodies, using a rapid immune\u2010chromatographic test. HCV RNA was quantified on anti\u2010HCV positive DBS, using the Abbott RealTime HCV\u00ae Genotyping was performed on DBS with detectable viral load with Versant\u00ae HCV Genotype 2.0 Assay (LiPA) and Abbott RealTime HCV Genotype II assay\u00ae. Among the 506 participants, 120 were tested as positive for anti\u2010HCV antibodies and their samples were analyzed for HCV RNA viral load and genotype. Out of the 120 DBS tested, HCV RNA was detected on 25 (20.8%). The median viral load was 15,058\u2009IU\/ml (ranging from 710 to 766,740\u2009IU\/ml). All positive DBS were suitable for the genotyping assay, that showed a predominance of genotype 1 (21\/25) including 16 genotypes 1a and 5 genotypes 1b. HCV genotype 1 prevails in a DU population in Dakar. DBS could be useful for HCV RNA genotyping, but optimal storage conditions should required avoiding RNA impairment. Acknowledging this limitation, DBS could be a great interest for detecting and genotyping HCV viremic patients.<jats:bold><jats:italic>J. Med. Virol. 89:484\u2013488, 2017<\/jats:italic>.<\/jats:bold>\u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24460","type":"journal-article","created":{"date-parts":[[2015,12,25]],"date-time":"2015-12-25T03:49:42Z","timestamp":1451015382000},"page":"484-488","source":"Crossref","is-referenced-by-count":2,"title":["Usefulness of Dried Blood Spots (DBS) to perform hepatitis C virus genotyping in drug users in Senegal"],"prefix":"10.1002","volume":"89","author":[{"given":"O.","family":"Ndiaye","sequence":"first","affiliation":[{"name":"Bacteriology\u2013Virology laboratory CHU A. le Dantec Cheikh Anta Diop University of Dakar Dakar Senegal"},{"name":"Regional center for research and training on HIV\/AIDS CHU Fann Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Gozlan","sequence":"additional","affiliation":[{"name":"Saint Antoine Hospital Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Diop\u2010Ndiaye","sequence":"additional","affiliation":[{"name":"Bacteriology\u2013Virology laboratory CHU A. le Dantec Cheikh Anta Diop University of Dakar Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.S.","family":"Sall","sequence":"additional","affiliation":[{"name":"Bacteriology\u2013Virology laboratory CHU A. le Dantec Cheikh Anta Diop University of Dakar Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Chapelain","sequence":"additional","affiliation":[{"name":"Saint Antoine Hospital Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Lepr\u00eatre","sequence":"additional","affiliation":[{"name":"Institute of Medecine and Applied Epidemiology\u2010IMEA Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Maynart","sequence":"additional","affiliation":[{"name":"Regional center for research and training on HIV\/AIDS CHU Fann Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Gueye","sequence":"additional","affiliation":[{"name":"Bacteriology\u2013Virology laboratory CHU A. le Dantec Cheikh Anta Diop University of Dakar Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Lo","sequence":"additional","affiliation":[{"name":"Bacteriology\u2013Virology laboratory CHU A. le Dantec Cheikh Anta Diop University of Dakar Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Thiam","sequence":"additional","affiliation":[{"name":"Bacteriology\u2013Virology laboratory CHU A. le Dantec Cheikh Anta Diop University of Dakar Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Ba","sequence":"additional","affiliation":[{"name":"Psychiatry Service, CHU Fann Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Lacombe","sequence":"additional","affiliation":[{"name":"Saint Antoine Hospital Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.M.","family":"Girard","sequence":"additional","affiliation":[{"name":"Saint Antoine Hospital Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Mboup","sequence":"additional","affiliation":[{"name":"Bacteriology\u2013Virology laboratory CHU A. le Dantec Cheikh Anta Diop University of Dakar Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.T.","family":"Kane","sequence":"additional","affiliation":[{"name":"Bacteriology\u2013Virology laboratory CHU A. le Dantec Cheikh Anta Diop University of Dakar Dakar Senegal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"3070","DOI":"10.1128\/JCM.36.10.3070-3072.1998","article-title":"Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature","volume":"36","author":"Abe K","year":"1998","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i17.2436"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2893.2000.00221.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2012.02.004"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2893.2002.00319.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annepidem.2014.09.002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.14.7914-7923.2003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01300-08"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-11-208"},{"key":"e_1_2_7_11_1","first-page":"47","article-title":"Seroprevalence of hepatitis C virus (HCV) in Senegalease blood donors","volume":"51","author":"Dieye TN","year":"2006","journal-title":"Dakar Med"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21236"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21492"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121873"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020047"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvh.12345"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268813000940"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp353"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-124-10-199605150-00002"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.7448\/IAS.18.1.19888"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03548-14"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00264-5"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-288"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26141"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21319"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20468"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.03.003"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2893.2005.00605.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000372"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01249-12"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3512-3514.2002"},{"key":"e_1_2_7_32_1","first-page":"165826","article-title":"Prevalence of hepatitis C virus genotypes in district bannu, khyber pakhtunkhwa, pakistan","volume":"2014","author":"Saleha S","year":"2014","journal-title":"Hepat Res Treat"},{"key":"e_1_2_7_33_1","doi-asserted-by":"crossref","first-page":"S35","DOI":"10.1002\/hep.1840360706","article-title":"Natural history of chronic hepatitis C","volume":"36","author":"Seeff LB","year":"2002","journal-title":"Hepatology"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70216-4"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23407"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1086\/380821"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02390-07"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.2.223-235.2000"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.24460","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.24460","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.24460","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T22:59:44Z","timestamp":1748732384000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.24460"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,9]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/jmv.24460"],"URL":"https:\/\/doi.org\/10.1002\/jmv.24460","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"type":"print","value":"0146-6615"},{"type":"electronic","value":"1096-9071"}],"subject":[],"published":{"date-parts":[[2016,11,9]]}}}