{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T10:00:51Z","timestamp":1788084051159,"version":"build-2784847793"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T00:00:00Z","timestamp":1454976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico - CNPq","award":["304872\/2011-3"],"award-info":[{"award-number":["304872\/2011-3"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa no Estado do Rio de Janeiro-FAPERJ","award":["E-26\/110.663\/2013"],"award-info":[{"award-number":["E-26\/110.663\/2013"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa no Estado do Rio de Janeiro-FAPERJ","award":["E\/-26\/103.149\/2011"],"award-info":[{"award-number":["E\/-26\/103.149\/2011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec><jats:label\/><jats:p>Dengue virus\u2010type 2 (DENV\u20102) caused three outbreaks, in the years 1990, 1998, and 2008, in Rio de Janeiro, Brazil. The 2008 outbreak was the most severe in reported cases, hospitalizations, and deaths. To investigate virological and epidemiological factors that may have contributed to the pathogenic profile of 2008 epidemic, 102 patients sera obtained during the epidemic and inter\u2010epidemic periods of three outbreaks were analysed by qRT\u2010PCR to estimate viremia levels and their correlation with the clinical, immunological, and demographic patient characteristics. DENV\u20102 isolates from the outbreaks were sequenced. Two DENV\u20102 lineages (I and II) of the American\/Asian genotype were confirmed, each exclusive for 1990\u20132002 and 2007\u20132011, respectively. The mean viremia level in the 2008 samples was two orders of magnitude higher than that of the 1990\u20132002 samples. Severe dengue cases increased from 31% in 1990\u20132002 to 69% in 2007\u20132011; in patients aged \u226415 years, from 3% in 1990\u20132002 to 37% in 2007\u20132011. The DENV\u20102 lineage II and younger age significantly contributed to the pathogenic profile of 2008 epidemic in Rio de Janeiro. <jats:bold><jats:italic>J. Med. Virol. 88:1130\u20131136, 2016<\/jats:italic><\/jats:bold>. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24464","type":"journal-article","created":{"date-parts":[[2016,1,6]],"date-time":"2016-01-06T09:31:09Z","timestamp":1452072669000},"page":"1130-1136","source":"Crossref","is-referenced-by-count":37,"title":["Dengue severity associated with age and a new lineage of dengue virus\u2010type 2 during an outbreak in Rio De Janeiro, Brazil"],"prefix":"10.1002","volume":"88","author":[{"given":"Priscila Conrado Guerra","family":"Nunes","sequence":"first","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone Alves Faria","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nieli","family":"Rodrigues da Costa","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Cesar Lima","family":"de Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique da Rocha Queiroz","family":"Lima","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saraiva Eliane M","family":"Araujo","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via Barreto","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaqueline Basto Santos","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca","family":"de Santis Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita Maria Ribeiro","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Maria Bispo","family":"de Filippis","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Foundation\/Oswaldo Cruz Institute Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.4269\/ajtmh.2006.75.1113","article-title":"Dengue hemorrhagic Fever caused by sequential dengue 1\u20103 virus infections over a long time interval: Havana epidemic, 2001\u20102002","volume":"75","author":"Alvarez M","year":"2006","journal-title":"Am J Trop Med Hyg"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020528"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.08.008"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059422"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002095"},{"key":"e_1_2_6_8_1","first-page":"82","article-title":"From primary care to hospitalization: Clinical warning signs of severe dengue fever in children and adolescents during an outbreak in Rio de Janeiro, Brazil","volume":"29","author":"Gibson G","year":"2013","journal-title":"Cad Saude Publica"},{"key":"e_1_2_6_9_1","first-page":"261","article-title":"Observations related to pathogenesis of dengue hemorrhagic fever. I. Experience with classification of dengue viruses","volume":"42","author":"Halstead SB","year":"1970","journal-title":"Yale J Biol Med"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.3.545-551.1992"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/trs091"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(99)00059-1"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1020-49892007001000009"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003084"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1603.090996"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.14393\/Hygeia1023174","article-title":"Uma an\u00e1lise espa\u00e7o\u2010temporal dos grupos de pesquisa do CNPQ: a dengue no Brasil","volume":"10","author":"Ramos R","year":"2014","journal-title":"Hygeia: Revista Brasileira de Geografia M\u00e9dica e da Sa\u00fade (Uberl\u00e2ndia)"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(03)59009-1"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8504"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011811"},{"key":"e_1_2_6_20_1","first-page":"40","article-title":"The pathogenesis of dengue haemorrhagic fever. A critical appraisal of current hypotheses","volume":"70","author":"Rosen L","year":"1986","journal-title":"S Afr Med J"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.09-0346"},{"key":"e_1_2_6_22_1","unstructured":"SVS (Secretaria de Vigil\u00e2ncia Sanit\u00e1ria).2012. Coordena\u00e7\u00e3o Geral do Programa Nacional de Dengue.http:\/\/portal.saude.gov.br\/portal\/arquivos\/pdf\/ano02_n03_dengue_brasil_si.pdf"},{"key":"e_1_2_6_23_1","unstructured":"SVS (Secretaria de Vigil\u00e2ncia Sanit\u00e1ria).2008. Relat\u00f3rio de casos de dengue.http:\/\/www.saude.rj.gov\/Docs\/Acoes\/dengue\/Relatorio.htm"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2010.98"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1086\/519170"},{"key":"e_1_2_6_26_1","unstructured":"SiqueiraJB Jr. VinhalLC SaidRFC HoffmannJL MartinsJ BarbirattoSB CoelhoGE.2010. Chapter 7. Dengue no Brasil: tend\u00eancias e mudan\u00e7as na epidemiologia com \u00eanfase nas epidemias de 2008 e 2010. In: Sa\u00fade Brasil 2010: uma an\u00e1lise da situa\u00e7\u00e3o de sa\u00fade e de evid\u00eancias selecionadas de impacto de a\u00e7\u00f5es de vigil\u00e2ncia em sa\u00fade. Minist\u00e9rio da Sa\u00fade Secretaria de Vigil\u00e2ncia em Sa\u00fade Departamento de An\u00e1lise de Situa\u00e7\u00e3o em Sa\u00fade. S\u00e9rie G. Estat\u00edstica e Informa\u00e7\u00e3o em Sa\u00fade. ISBN 978\u201085\u2010334\u20101851\u20106. Bras\u00edlia: Minist\u00e9rio da Sa\u00fade 372 p. Available:http:\/\/bvsms.saude.gov.br\/bvs\/publicacoes\/saude_brasil_2010.pdf"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-311X2009001300002"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2008.78.990"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1086\/315215"},{"key":"e_1_2_6_31_1","first-page":"e57 2010","article-title":"Emergence of the Asian 1 genotype of dengue virus serotype 2 in viet nam: In vivo fitness advantage and lineage replacement in South\u2010East Asia","volume":"4","author":"Vu TT","journal-title":"PLoS Negl Trop Dis"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1704"},{"key":"e_1_2_6_33_1","first-page":"3","volume-title":"Dengue guidelines for diagnosis, treatment, prevention and control","year":"2009"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.24464","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.24464","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T05:10:33Z","timestamp":1696396233000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.24464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,9]]},"references-count":32,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1002\/jmv.24464"],"URL":"https:\/\/doi.org\/10.1002\/jmv.24464","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,9]]}}}