{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:55:31Z","timestamp":1791320131003,"version":"4.3.1"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T00:00:00Z","timestamp":1459123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500169"],"award-info":[{"award-number":["81500169"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81272182"],"award-info":[{"award-number":["81272182"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005225","name":"Construct Program of the Key Discipline in Hunan Province","doi-asserted-by":"publisher","award":["(2011\u201376)"],"award-info":[{"award-number":["(2011\u201376)"]}],"id":[{"id":"10.13039\/501100005225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec><jats:label\/><jats:p>Epstein\u2013Barr virus (EBV) is a human oncogenic herpesvirus associated with lymphoma and nasopharyngeal carcinoma. Because the susceptible hosts of EB virus are limited to human and cotton\u2010top tamarins (Saguinus oedipus), there have been no appropriate animal models until the lymphoma model induced by EBV in human peripheral blood lymphocyte (hu\u2010PBL)\/SCID chimeric mice was reported. However, it is still controversial whether the EBV\u2010associated lymphoma induced in hu\u2010PBL\/SCID mice is a monoclonal tumor. In this study, we transplanted normal human peripheral blood lymphocytes (hu\u2010PBL) from six donors infected with EBV into SCID mice to construct hu\u2010PBL\/SCID chimeric mice. The induced tumors were found in the mediastinum or abdominal cavity of SCID mice. Microscopic observation exhibited tumor cells that were large and had a plasmablastic, centroblastic or immunoblastic\u2010like appearance. Immunophenotyping assays showed the induced tumors were LCA\u2010positive, CD20\/CD79a\u2010positive (markers of B cells), and CD3\/CD45RO\u2010negative (markers of T cells). A human\u2010specific <jats:italic>Alu<\/jats:italic> sequence could be amplified by <jats:italic>Alu<\/jats:italic>\u2010PCR. This confirmed that induced tumors were B\u2010cell lymphomas originating from the transplanted human lymphocytes rather than mouse cells. EBER in situ hybridization detected positive signals in the nuclei of the tumor cells. Expression of EBV\u2010encoded LMP1, EBNA\u20101, and EBNA\u20102 in the tumors was significantly positive. PCR\u2010based capillary electrophoresis analysis of IgH gene rearrangement revealed a monoclonal peak and single amplification product in all six cases of induced tumors. This indicated that EBV can induce monoclonal proliferation of human B lymphocytes and promotes the development of lymphoma. <jats:bold><jats:italic>J. Med. Virol. 88:1804\u20131813, 2016<\/jats:italic><\/jats:bold>. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24526","type":"journal-article","created":{"date-parts":[[2016,3,19]],"date-time":"2016-03-19T06:58:55Z","timestamp":1458370735000},"page":"1804-1813","source":"Crossref","is-referenced-by-count":7,"title":["Identification of IgH gene rearrangement and immunophenotype in an animal model of Epstein\u2013Barr virus\u2010associated lymphomas"],"prefix":"10.1002","volume":"88","author":[{"given":"Yang","family":"Zhang","sequence":"first","affiliation":[{"name":"Cancer Research Institute College of Medicine University of South China Chang Sheng Xi Avenue 28 Hengyang Hunan 421001 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqin","family":"Peng","sequence":"additional","affiliation":[{"name":"Cancer Research Institute College of Medicine University of South China Chang Sheng Xi Avenue 28 Hengyang Hunan 421001 P.R. 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