{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:52:50Z","timestamp":1790031170077,"version":"4.0.1"},"reference-count":51,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,5,30]],"date-time":"2016-05-30T00:00:00Z","timestamp":1464566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>There is limited information on the variations of HIV\u20101 DNA mutation profile in reverse transcriptase (RT) and protease (PR) genes during suppressive antiretroviral treatment (plasma HIV\u20101 RNA continuously &lt;50\u2009copies\/ml) with raltegravir (RAL)\u2010based regimens in patients with baseline RT\/PR resistant HIV. Twelve multidrug resistant (RT: 12\/12, PR: 8\/12) HIV\u2010infected patients were followed during effectively suppressive RAL\u2010based therapy. Total and integrated HIV\u20101 DNA were assessed by real time PCR at baseline and every 6 months. Ultrasensitive (threshold: 2.5\u2009copies\/ml) plasma HIV\u20101 RNA and genotypic analysis of RT and PR in proviral DNA were performed at baseline and at 24 months. Half of the patients had full viral suppression (plasma HIV\u2010RNA\u2009&lt;\u20092.5\u2009copies\/ml) at month 12. Total HIV\u20101 DNA declined significantly after 12 months of therapy (from 249.2 to 145.7\u2009copies\/10<jats:sup>6<\/jats:sup>cells,<jats:italic>P<\/jats:italic>\u2009=\u20090.023), and remained stable until 24 months, when total HIV\u20101 DNA levels raised, concomitantly with a less stringent suppression of HIV\u20101 RNA (81.8% of patients with &gt;2.5\u2009copies\/ml). Integrated HIV\u20101 DNA did not show fluctuations during the study period. Sequencing of the PR and RT regions from HIV\u20101 DNA revealed changes in the resistance mutation profile in five patients. Total HIV\u20101 DNA declined after the introduction of RAL\u2010based therapy, with a rebound after 2 years. No changes were observed in levels of integrated DNA, suggesting limited effect on archived HIV. The RT and PR sequence changes in archived HIV\u20101 DNA suggest that variation of the mutation profile can occur even in the absence of detectable HIV\u20101 RNA.<jats:bold><jats:italic>J. Med. Virol. 88:2115\u20132124, 2016<\/jats:italic>.<\/jats:bold>\u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24581","type":"journal-article","created":{"date-parts":[[2016,5,21]],"date-time":"2016-05-21T13:17:21Z","timestamp":1463836641000},"page":"2115-2124","source":"Crossref","is-referenced-by-count":7,"title":["HIV\u20101 DNA dynamics and variations in HIV\u20101 DNA protease and reverse transcriptase sequences in multidrug\u2010resistant patients during successful raltegravir\u2010based therapy"],"prefix":"10.1002","volume":"88","author":[{"given":"Zuleika","family":"Michelini","sequence":"first","affiliation":[{"name":"Department of Therapeutic Research and Medicines Evaluation Istituto Superiore di Sanit\u00e0 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clementina Maria","family":"Galluzzo","sequence":"additional","affiliation":[{"name":"Department of Therapeutic Research and Medicines Evaluation Istituto Superiore di 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