{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:20:39Z","timestamp":1760955639251},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T00:00:00Z","timestamp":1468800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:label \/><jats:p>To establish a dual fluorescence quantitative real\u2010time polymerase chain reaction (DFqRT\u2010PCR) method for typing inactivated bivalent vaccines of Hantaan virus (HTNV, type I) and Seoul virus (SEOV, type II) found in China. Primers and probes were designed based on S segment of the Hantavirus. FAM and HEX were added to the probes for the type I and type II vaccines, respectively. The S fragments were cloned into the pMD18\u2010T vector to create pMD18\u2010T\/HTNV and pMD18\u2010T\/SEOV plasmids, respectively. The DFqRT\u2010PCR reaction conditions were optimized. The specificity, sensitivity, accuracy, and reproducibility of the DFqRT\u2010PCR method within and among batches of the samples and labs were evaluated. The sensitivity of the DFqRT\u2010PCR method for detecting type I and II vaccines was 4.68\u2009\u00d7\u200910<jats:sup>1<\/jats:sup> copies\/\u00b5L and 5\u2009\u00d7\u200910<jats:sup>1<\/jats:sup> copies\/\u00b5L of pMD18\u2010T\/HTNV and pMD18\u2010T\/SEOV, respectively. This method distinguished the hantavirus vaccines from several other virus genus and distinguished the type I and II vaccines from each other with coefficients of variation (CVs) of the intra\u2010 and inter\u2010batch assay of 0.19\u20130.66% and 0.09\u20130.69%, respectively. For bivalent inactivated vaccine, the CVs of the intra\u2010 and inter\u2010batch assays, and the intra\u2010 and inter\u2010laboratory assays were 0.06\u20131.82% for the samples from the same batches and different batches from the same manufacturer or the samples from the different manufacturers. The DFqRT\u2010PCR method was simple, fast, and sensitive for identifying and determining the inactivated hantavirus vaccine with high accuracy and reproducibility. Therefore, it can be used for quality control of hantavirus vaccines. <jats:bold><jats:italic>J. Med. Virol. 89:10\u201316, 2017<\/jats:italic><\/jats:bold>. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24599","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T17:42:45Z","timestamp":1466012565000},"page":"10-16","source":"Crossref","is-referenced-by-count":3,"title":["Type inactivated bivalent hantavirus vaccines using dual fluorescence quantitative real\u2010time PCR: Establishment and evaluation"],"prefix":"10.1002","volume":"89","author":[{"given":"Wei","family":"Jiang","sequence":"first","affiliation":[{"name":"Changchun Institute of Biological Products Co. Ltd. Changchun P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Ma","sequence":"additional","affiliation":[{"name":"Beijing Kawin Technology Share\u2010Holding Co. Ltd. Beijing P.R. 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