{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T08:49:56Z","timestamp":1784191796245,"version":"3.55.0"},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T00:00:00Z","timestamp":1467763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Tunisian Ministry for Scientific Research and Technology","award":["LR11-IPT09"],"award-info":[{"award-number":["LR11-IPT09"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec><jats:label\/><jats:p>Human adenoviruses (HAdVs) are common causes of conjunctivitis. This study describes the epidemiological features and characterizes by phylogenetic analysis HAdVs isolated from patients with conjunctivitis in Tunisia, North Africa. Data on out\u2010patients presenting with conjunctivitis during 2 years (2012\u20132013) were analyzed. Conjunctival swabs obtained from 240 patients were assessed for the presence of HAdVs by PCR amplification on the fiber and hexon genes. Positive PCR products, together with those of nine viral isolates from previous years, were sequenced and analyzed phylogenetically. Conjunctivitis represented 11.5% of all reasons of consultations with a slight increase between mid\u2010March and mid\u2010June. Sixty\u2010five percent of samples (n\u2009=\u2009156) revealed positive by at least one PCR test. PCR amplification in the hexon gene was slightly more sensitive as compared to the fiber gene. Genotyping in the two genomic regions gave concordant results for almost all isolates. HAdV\u2010D8 was the most predominant genotype (87.6%) and was detected continuously from 2000 to 2013. Minor co\u2010circulating genotypes including HAdV\u2010E4, HAdV\u2010B3, HAdV\u2010B55, and HAdV\u2010D37 were identified; most of them were detected by amplification in the hexon gene. In conclusion, this work reports molecular data on adenoviral conjunctivitis from a region where such information is scarce and contributes to a better knowledge of the worldwide distribution of causative genotypes. It revealed a predominance and endemic circulation of HAdV\u2010D8, a genotype that was mainly reported from epidemic keratoconjunctivitis. It shows that PCR amplification in two different genomic regions enhances the sensitivity of HAdV detection in clinical samples and the identification of minor genotypes. <jats:bold><jats:italic>J. Med. Virol. 89:304\u2013312, 2017<\/jats:italic>.<\/jats:bold> \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24622","type":"journal-article","created":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T11:10:19Z","timestamp":1467371419000},"page":"304-312","source":"Crossref","is-referenced-by-count":8,"title":["Molecular detection and characterization through analysis of the hexon and fiber genes of Adenoviruses causing conjunctivitis in Tunisia, North Africa"],"prefix":"10.1002","volume":"89","author":[{"given":"Nadia","family":"Fedaoui","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Virology Pasteur Institute of Tunis Tunis Tunisia"},{"name":"Faculty of Sciences of Bizerte University of Carthage Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narjess","family":"Ben Ayed","sequence":"additional","affiliation":[{"name":"Institute of Ophtalmology Hedi Raies Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahlem","family":"Ben Yahia","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Virology Pasteur Institute of Tunis Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walid","family":"Hammami","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Virology Pasteur Institute of Tunis Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Matri","sequence":"additional","affiliation":[{"name":"Institute of Ophtalmology Hedi Raies Tunis Tunisia"},{"name":"University of Tunis El Manar Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Nacef","sequence":"additional","affiliation":[{"name":"Institute of Ophtalmology Hedi Raies Tunis Tunisia"},{"name":"University of Tunis El Manar Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henda","family":"Triki","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Virology Pasteur Institute of Tunis Tunis Tunisia"},{"name":"University of Tunis El Manar Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.56.2.120"},{"key":"e_1_2_7_3_1","first-page":"2010","article-title":"Rapid detection and typing of oculopathogenic strain of subgenus D adenoviruses by fiber\u2010based PCR and restriction enzyme analysis","volume":"42","author":"Adhikary AK","year":"2001","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"19707","DOI":"10.2807\/ese.15.45.19707-en","article-title":"Increasing case numbers of adenovirus conjunctivitis in Germany, 2010","volume":"15","author":"Adlhoch C","year":"2010","journal-title":"Euro Surveill"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10384-010-0894-x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004397-200201000-00008"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.2.726-732.2005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2010.04.008"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.13-1186"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.62.12.843"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-007-9054-z"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.65.457"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24071"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.65.260"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1556\/EuJMI.4.2014.1.2"},{"key":"e_1_2_7_16_1","first-page":"539","article-title":"Surveillance and control of epidemic keratoconjunctivitis","volume":"94","author":"Gottsch JD","year":"1996","journal-title":"Trans Am Ophthalmol Soc"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-85-20825"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.66.436"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110781"},{"key":"e_1_2_7_20_1","first-page":"342","article-title":"Recent advance in new types of human adenovirus","volume":"29","author":"Huang GH","year":"2013","journal-title":"Bing Du Xue Bao"},{"key":"e_1_2_7_21_1","unstructured":"Human adenovirus working group. 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