{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:44:10Z","timestamp":1791873850350,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,8,9]],"date-time":"2016-08-09T00:00:00Z","timestamp":1470700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de S\u00e3o Paulo","award":["2010\/10619- 0"],"award-info":[{"award-number":["2010\/10619- 0"]}]},{"name":"MsC CAPES scholarship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>Patients undergoing Natalizumab (NTZ) therapy are at risk of progressive multifocal leukoencephalopathy (PML). Besides John Cunningham virus (JCV), BK polyomavirus might represent an additional concern for such patients since it can also infect CNS cells. Currently, data regarding the presence of anti\u2010JCV antibodies added to previous immunosuppressive therapy and prolonged NTZ therapy has been used to classify patients at risk of developing PML. Here, we investigated the profile shedding of JCV and BKV in multiple sclerosis (MS) patients during treatment with NTZ. Serial blood and urine samples from 97 MS patients receiving either NTZ or \u03b2\u2010interferon were investigated for polyomavirus shedding. While all blood samples tested negative, 36% of the patients shed polyomavirus in the urine in at least one time point. From these, 21.7%, 9.3%, and 5.1% shed JCV, BKV, and both polyomavirus, respectively. No difference was observed between the rates of urinary shedding of patients treated with NTZ (38.9%) and patients treated with other drugs (34.5%), also no PML event was diagnosed during the follow\u2010up. Therefore, urinary shedding might not be interfered by therapy condition. In our study, we also observed 14\/27 (52%) of anti\u2010JCV antibodies prevalence, and nearly half of them (42%) did not present any event of urinary shedding during the follow\u2010up. <jats:bold><jats:italic>J. Med. Virol. 89:528\u2013534, 2017<\/jats:italic>.<\/jats:bold> \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24646","type":"journal-article","created":{"date-parts":[[2016,7,28]],"date-time":"2016-07-28T07:42:47Z","timestamp":1469691767000},"page":"528-534","source":"Crossref","is-referenced-by-count":4,"title":["Polyomavirus detection in multiple sclerosis patients under natalizumab therapy: Profile and frequency of urinary shedding"],"prefix":"10.1002","volume":"89","author":[{"given":"Luiz Henrique","family":"Nali","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Virologia Instituto de Medicina Tropical de S\u00e3o Paulo Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Fink","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Instituto de Medicina Tropical de S\u00e3o Paulo Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme S.","family":"do Olival","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Instituto de Medicina Tropical de S\u00e3o Paulo Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Departamento de Neurologia Santa Casa de Miseric\u00f3rdia de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenira","family":"Moraes","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas de S\u00e3o Paulo\u2014HCFMUSP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dagoberto","family":"Callegaro","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas de S\u00e3o Paulo\u2014HCFMUSP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles Peter","family":"Tilbery","sequence":"additional","affiliation":[{"name":"Departamento de Neurologia Santa Casa de Miseric\u00f3rdia de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Ernesto","family":"Vidal","sequence":"additional","affiliation":[{"name":"Grupo de Neuroci\u00eancias Instituto de Infectologia Em\u00edlio Ribas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Masami","family":"Sumita","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Instituto de Medicina Tropical de S\u00e3o Paulo Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augusto C. 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