{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T16:22:35Z","timestamp":1780590155668,"version":"3.54.1"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,2,27]],"date-time":"2017-02-27T00:00:00Z","timestamp":1488153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81400096"],"award-info":[{"award-number":["81400096"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:sec><jats:label\/><jats:p>Background: Severe fever with thrombocytopenia syndrome bunyavirus (sftsv) is an emerging tick\u2010borne rna virus recently identified as the pathogen that causes severe fever with thrombocytopenia syndrome (sfts) in china. the existing commercial nucleic acid testing (comnat) assay with a relatively high claimed limit of quantitative detection (loqd) is not capable of sensitive detection and quantitation of sftsv. Thus, a new real\u2010time reverse transcriptase (rt)\u2010pcr assay with improved sensitivity is needed for clinical diagnosis; it could also be used to screen blood donors if necessary. Materials and methods: We developed a new sftsv rt\u2010pcr nat assay (newnat). About 129 plasma samples from 93 suspected sfts patients with typical clinical symptoms were tested using an anti\u2010sftsv total antibody elisa and both comnat and newnat. The test performance of the two nat assays was evaluated and compared. Results: The newnat had a lower limit for quantitative testing compared to comnat. Twelve samples were comnat negative but newnat positive. Out of 35 suspected sfts patients who were comnat negative and anti\u2010sftsv total antibody negative, four tested positive by the newnat assay and one of these four seroconverted within 2\u20134 days after testing newnat positive. A high correlation was observed between the cts of the newnat and comnat assays. Conclusion: The newnat assay was sensitive for quantitative detection of sftsv and may be applicable to clinical diagnosis and studies of the need for blood donor screening.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24760","type":"journal-article","created":{"date-parts":[[2016,12,30]],"date-time":"2016-12-30T16:07:42Z","timestamp":1483114062000},"page":"1131-1138","source":"Crossref","is-referenced-by-count":13,"title":["Development and validation of a real\u2010time reverse transcriptase PCR assay for sensitive detection of SFTSV"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6668-2380","authenticated-orcid":false,"given":"Peibin","family":"Zeng","sequence":"first","affiliation":[{"name":"West China School of Public Health Sichuan University Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhendong","family":"Yang","sequence":"additional","affiliation":[{"name":"Xinyang 154 Military Hospital Xinyang 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