{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:14:51Z","timestamp":1740104091410,"version":"3.37.3"},"reference-count":56,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T00:00:00Z","timestamp":1493683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["15fk0210019h0003"],"award-info":[{"award-number":["15fk0210019h0003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:label\/><jats:p>Recent genome\u2010wide studies have demonstrated that HLA class II gene may play an important role in viral hepatitis. We studied genetic polymorphism and RNA expression of HLA class II genes in HCV\u2010related liver diseases. The study was performed in groups consisting of 24 patients with HCV\u2010related liver disease (12 of persistent normal ALT: PNALT group and 12 of advanced liver disease: ALD group) and 26 patients without HCV infection (control group). In PBMC samples, RNA expression of HLA class II genes (<jats:italic>HLA\u2010DPA1<\/jats:italic>, <jats:italic>DPB1<\/jats:italic>, <jats:italic>DQA1<\/jats:italic>, <jats:italic>DQB1<\/jats:italic>, and <jats:italic>DRB1<\/jats:italic>) was analyzed by real\u2010time RT\u2010PCR. Furthermore, 22 single nucleotide polymorphisms (SNPs) in HLA class II gene and two SNPs in IL28B gene were genotyped by genetic analyzer (GENECUBE\u00ae). In expression analysis, only DPB1 level was significantly different. Mean expression level of DPB1gene in control group was 160.0, PNALT group 233.8, and ALD group 465.0 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). Of 24 SNPs, allele frequencies were statistically different in two SNPs (rs2071025 and rs3116996) between PNALT groups and ALD group (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). In rs2071025, TT genotype was frequently detected in ALD group and expression level was significantly higher than the other genotypes (449.2 vs 312.9, <jats:italic>P\u2009<\/jats:italic>&lt;\u20090.01). In rs3116996, TA or TT (non AA) genotype was frequently detected in ALD group and expression level was significantly higher than genotype AA (457.1 vs 220.9, <jats:italic>P\u2009<\/jats:italic>&lt;\u20090.01). Genotyping and expression analysis in HLA class II gene revealed that two SNPs of HLA\u2010DPB1 (rs2071025 and rs3116996) were significantly correlated to RNA expression and progression of HCV\u2010related liver diseases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24814","type":"journal-article","created":{"date-parts":[[2017,3,23]],"date-time":"2017-03-23T23:18:26Z","timestamp":1490311106000},"page":"1574-1583","source":"Crossref","is-referenced-by-count":3,"title":["Identification of novel variants in HLA class II region related to HLA DPB1 expression and disease progression in patients with chronic hepatitis C"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1377-8455","authenticated-orcid":false,"given":"Katsushi","family":"Hiramatsu","sequence":"first","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0860-1199","authenticated-orcid":false,"given":"Hidetaka","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Nemoto","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuto","family":"Nosaka","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Saito","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsushi","family":"Naito","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuto","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuya","family":"Ofuji","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Ohtani","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Suto","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Second Department of Internal Medicine University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiro","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Division of Medical Genetics and Biochemistry University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Hida","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Department of Clinical Laboratories University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideki","family":"Kimura","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, Department of Clinical Laboratories University of Fukui Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Soya","sequence":"additional","affiliation":[{"name":"Tsuruga Institute of Biotechnology Toyobo Co., Ltd. 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