{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T10:12:38Z","timestamp":1768558358851,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2014ZX09102044\u2010007"],"award-info":[{"award-number":["2014ZX09102044\u2010007"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2015ZX09102025"],"award-info":[{"award-number":["2015ZX09102025"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2016T90637"],"award-info":[{"award-number":["2016T90637"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>Marburg virus (MARV), which is one of the most virulent agents in the world, causes lethal haemorrhagic fever in humans and nonhuman primates (NHPs) with a mortality rate of up to 90%. Currently, there is no effective treatment or approved vaccine for MARV for human use to control disease outbreak and spread. Virus\u2010like particles (VLPs), which are morphologically identical to the native infectious virus particle, are efficacious as vaccines against many viruses, including human papilloma virus (HPV), porcine circovirus (PCV) type 2 and hepatitis B virus (HBV). In this study, we generated MARV virus\u2010like particles (VLPs) by co\u2010expressing a glycoprotein (GP) and matrix protein (VP40) using the baculovirus expression system. Rhesus macaques vaccinated with MARV VLPs mixed with adjuvant Poria cocos polysaccharides (PCP\u2010II) produced a GP\u2010specific IgG titer of up to 1:1280 and virus\u2010neutralizing antibody titers that reached 1:320. MARV VLPs also elicited interferon\u2010\u03b3 (IFN\u2010\u03b3) and interleukin\u20104 (IL\u20104) secretion associated with T\u2010helper 1 cell (Th1)\u2010 and T\u2010helper 2 cell (Th2)\u2010mediated immunity, as detected using enzyme\u2010linked immunospot (ELISpot) assays. These data indicate that MARV VLPs mixed with adjuvant PCP\u2010II have excellent immunogenicity in rhesus macaques and may be a promising candidate vaccine against MARV.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24832","type":"journal-article","created":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T11:47:16Z","timestamp":1491997636000},"page":"2069-2074","source":"Crossref","is-referenced-by-count":7,"title":["Marburg virus\u2010like particles produced in insect cells induce neutralizing antibodies in rhesus macaques"],"prefix":"10.1002","volume":"89","author":[{"given":"Gai","family":"Weiwei","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Jilin University Changchun China"},{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control Institute of Military Veterinary Academy of Military Medical Sciences Changchun 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