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Type I IFNs signal through Jak\/STAT pathway leading to the production of a few hundred interferon stimulated genes (ISGs). The aim of this study was to explore the role of one of these ISGs, MxA in HCV infection and type I IFN production. Plasmid encoding MxA was cloned into PcDNA3.1\u20103\u00d7tag vector and MxA expression was confirmed both at mRNA (RT\u2010PCR) and protein (Western blot, WB) levels. IFN\u03b1 and IFN\u03b2 productions were quantified by RT\u2010PCR from cell lysate and by ELISA kit from culture medium following MxA over\u2010expression in Huh7.5.1 cells. The activation status of Jak\/STAT signaling pathway was examined at three levels: p\u2010STAT1 (WB), interferon sensitive response element (ISRE) activity (dual luciferase reporter gene assay), and levels of ISG expression (RT\u2010qPCR). J6\/JFH1 HCV culture system was used to study the role of MxA in HCV replication. Our findings indicated that MxA over\u2010expression inhibited HCV replication and potentiated the IFN\u03b1\u2010mediated anti\u2010HCV activity; MxA stimulated the production of IFN\u03b1, IFN\u03b2, and enhanced IFN\u03b1\u2010induced activation of Jak\u2010STAT signaling pathway. We concluded that MxA is a positive regulator of type I IFN signaling in HCV infection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24867","type":"journal-article","created":{"date-parts":[[2017,5,31]],"date-time":"2017-05-31T10:34:08Z","timestamp":1496226848000},"page":"2173-2180","source":"Crossref","is-referenced-by-count":24,"title":["MxA is a positive regulator of type I IFN signaling in HCV infection"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6709-0202","authenticated-orcid":false,"given":"Xuezhen","family":"Shi","sequence":"first","affiliation":[{"name":"Institute of Blood Transfusion Chinese Academy of Medical Sciences and Peking Union Medical College Chengdu Sichuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baihai","family":"Jiao","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion Chinese Academy of Medical Sciences and Peking Union Medical College Chengdu Sichuan 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