{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T16:55:28Z","timestamp":1783875328717,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T00:00:00Z","timestamp":1503964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>With the promotion of inactivated poliomyelitis vaccine (IPV) and live attenuated oral poliomyelitis vaccine (OPV), the global reported cases of poliomyelitis have reduced sharply from 0.35 million in 1988 to 74 in 2015. The Polio Eradication &amp; Endgame Strategic Plan published by WHO in 2013 included the strategy of implementation of poliovirus safe handling and containment measures to minimize the risks of facility\u2010associated reintroduction of virus into the polio\u2010free community to prevent the re\u2010import of poliovirus. Toward this strategy, we produced replication\u2010incompetent pseudovirus of poliovirus type 1, 2, 3 attenuated strains by constructing poliovirus capsid expression vectors and poliovirus replicon then transfecting HEK293T cells and developed a pseudovirus\u2010based neutralization assay (pNA) to determine neutralizing antibody titer which is more secure, time\u2010saving and reliable than conventional neutralization assay (cNA). By using anti\u2010poliovirus rat serum, we demonstrated excellent correlation between neutralizing antibody titers measured by cNA and pNA. It was concluded that pNA can be a potential alternative to replace cNA as a safe and time\u2010saving system for titer determination after live poliovirus's safekeeping.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24909","type":"journal-article","created":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T09:16:38Z","timestamp":1502183798000},"page":"2075-2083","source":"Crossref","is-referenced-by-count":12,"title":["A safe and reliable neutralization assay based on pseudovirus to measure neutralizing antibody titer against poliovirus"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4669-2703","authenticated-orcid":false,"given":"Shaohua","family":"Liu","sequence":"first","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Song","sequence":"additional","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Bai","sequence":"additional","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Lu","sequence":"additional","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxian","family":"Dai","sequence":"additional","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunsheng","family":"Hao","sequence":"additional","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijie","family":"Guo","sequence":"additional","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuling","family":"Li","sequence":"additional","affiliation":[{"name":"National Vaccine and Serum Institute Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"555","volume-title":"Viral Pathogenesis","author":"Minor PD","year":"1997"},{"key":"e_1_2_7_3_1","unstructured":"Polio vaccines: WHO position paper January 2014 Weekly Epidemiological Record 28 February 2014 vol. 89 9 (pp. 73\u201392).http:\/\/www.who.int\/wer\/2014\/wer8909.pdf"},{"key":"e_1_2_7_4_1","first-page":"285","article-title":"Disease eradication as a public health strategy: a case study of poliomyelitis eradication","volume":"78","author":"Aylward RB","year":"2000","journal-title":"Bull World Health Organ"},{"key":"e_1_2_7_5_1","unstructured":"Recommendations to assure the quality safety and efficacy of 11 poliomyelitis vaccine (inactivated) Geneva 13 to 17 October 2014 World Health Organization http:\/\/101.96.8.165\/www.who.int\/biologicals\/IPV_FINAL_for_BS2233_07072014(2).pdf"},{"key":"e_1_2_7_6_1","unstructured":"WHO Global Action Plan for Laboratory Containment of Wild Polioviruses. 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