{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:30:08Z","timestamp":1787495408505,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T00:00:00Z","timestamp":1505347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>There are many varieties of gastroenteritis viruses, of which norovirus (NoV) accounts for over 90% of the viral food poisoning incidents in Japan. However, protocols for rapidly identifying other gastroenteritis viruses need to be established to investigate NoV\u2010negative cases intensively. In this study, a multiplex real\u2010time PCR assay targeting rotavirus A, rotavirus C, sapovirus, astrovirus, adenovirus, and enterovirus was developed using stool samples collected from gastroenteritis patients between 2010 and 2013 in Fukui Prefecture, Japan. Of the 126 samples collected sporadically from pediatric patients with suspected infectious gastroenteritis, 51 were positive for non\u2010NoV target viruses, whereas 27 were positive for NoV, showing a high prevalence of non\u2010NoV viruses in pediatric patients. In contrast, testing in 382 samples of 58 gastroenteritis outbreaks showed that non\u2010NoV viruses were detected in 13 samples, with NoV in 267. Of the 267 NoV\u2010positive patients, only two were co\u2010infected with non\u2010NoV target viruses, suggesting that testing for non\u2010NoV gastroenteritis viruses in NoV\u2010positive samples was mostly unnecessary in outbreak investigations. Given these results, multiplex real\u2010time PCR testing for non\u2010NoV gastroenteritis viruses, conducted separately from NoV testing, may be helpful to deal with two types of epidemiological investigations, regular surveillance of infectious gastroenteritis and urgent testing when gastroenteritis outbreaks occur.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.24926","type":"journal-article","created":{"date-parts":[[2017,8,28]],"date-time":"2017-08-28T06:08:53Z","timestamp":1503900533000},"page":"67-75","source":"Crossref","is-referenced-by-count":14,"title":["Development of a multiplex real\u2010time PCR assay for detection of human enteric viruses other than norovirus using samples collected from gastroenteritis patients in Fukui Prefecture, Japan"],"prefix":"10.1002","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8337-9815","authenticated-orcid":false,"given":"Kazuaki","family":"Kowada","sequence":"first","affiliation":[{"name":"Fukui Prefectural Institute of Public Health and Environmental Science Fukui Japan"},{"name":"University of Fukui Fukui Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"University of Fukui Fukui Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiko","family":"Hirano","sequence":"additional","affiliation":[{"name":"Fukui Prefectural Institute of Public Health and Environmental Science Fukui Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Toho","sequence":"additional","affiliation":[{"name":"Fukui Prefectural Institute of Public Health and Environmental Science Fukui Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyonao","family":"Sada","sequence":"additional","affiliation":[{"name":"University of Fukui Fukui Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.07.050054"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.qco.0000171922.55075.ad"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200410000-00011"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24387"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/160.4.611"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23992"},{"key":"e_1_2_7_8_1","unstructured":"Ministry of Health Labour and Welfare of Japan. 2011; Food Poisoning Statistics 2009.https:\/\/www.mhlw.go.jp\/english\/topics\/foodsafety\/poisoning\/dl\/Food_Poisoning_Statistics_2009.pdf."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-006-0820-1"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1310.070402"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.28.2.276-282.1990"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9071(200007)61:3<326::AID-JMV7>3.0.CO;2-W"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01603-06"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00542-06"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1548-1557.2003"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00404-9"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.03.006"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"3185","DOI":"10.1128\/jcm.34.12.3185-3189.1996","article-title":"Molecular analysis of outer capsid glycoprotein (VP7) genes from two isolates of human group","volume":"34","author":"Kuzuya M","year":"1996","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2010.07.019"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12107"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis028"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24445"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24155"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/v8020042"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.533"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-011-1006-z"},{"key":"e_1_2_7_27_1","unstructured":"Centers for Disease Control and Prevention (CDC). 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