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In this report, a total of 684 cervical specimens from women diagnosed with vaginitis were assessed by the HR HPV RT\u2010PCR and the PCR reaction and reverse dot blot (PCR\u2010RDB) assays, using a PCR\u2010sequencing method as a reference standard. A total coincidence of 97.7% between the HR HPV RT PCR and the PCR\u2010RDB assays was determined with a Kappa value of 0.953. The HR HPV RT PCR assay had sensitivity, specificity, and concordance rates (accuracy) of 99.7%, 99.7%, and 99.7%, respectively, as confirmed by PCR\u2010sequencing, while the PCR\u2010RDB assay had respective rates of 98.8%, 97.1%, and 98.0%. The overall rate of HPV infection, determined by PCR\u2010sequencing, in women diagnosed with vaginitis was 49.85%, including 36.26% of single infection and 13.6% of multiple infections. The most common infections among the 15 high\u2010risk HPV types in women diagnosed with vaginitis were HPV\u201052, HPV\u201016, and HPV\u201058, with a total detection rate of 10.23%, 7.75%, and 5.85%, respectively. We conclude that the HR HPV RT PCR assay exhibits better clinical performance than the PCR\u2010RDB assay, and is an ideal alternative method for HPV genotyping. In addition, the HR HPV RT PCR assay provides HPV DNA viral loads, and could serve as a quantitative marker in the diagnosis and treatment of single and multiple HPV infections.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24931","type":"journal-article","created":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T16:05:59Z","timestamp":1504022759000},"page":"177-183","source":"Crossref","is-referenced-by-count":18,"title":["Comparison of the performance in detection of HPV infections between the high\u2010risk HPV genotyping real time PCR and the PCR\u2010reverse dot blot assays"],"prefix":"10.1002","volume":"90","author":[{"given":"Lahong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine The Affiliated Hospital of Hangzhou Normal University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibei","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The Second 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