{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:40:27Z","timestamp":1773283227270,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,11,3]],"date-time":"2017-11-03T00:00:00Z","timestamp":1509667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CES12\/003"],"award-info":[{"award-number":["CES12\/003"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CM13\/0309"],"award-info":[{"award-number":["CM13\/0309"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FI14\/0264"],"award-info":[{"award-number":["FI14\/0264"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["ICI14\/00372"],"award-info":[{"award-number":["ICI14\/00372"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["Pi13\/01574"],"award-info":[{"award-number":["Pi13\/01574"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>Oral DAA have demonstrated high efficacy as treatment of hepatitis C. However, the presence of resistance\u2010associated substitutions (RAS) at baseline has occasionally been associated with impaired treatment response. Herein, we examined the impact of baseline RAS at the HCV NS5A gene region on treatment response in a real\u2010life setting. All hepatitis C patients treated with DAA including NS5A inhibitors at our institution were retrospectively examined. The virus NS5A gene was analyzed using population sequencing at baseline and after 24 weeks of completing therapy in all patients that failed. All changes recorded at positions 28, 29, 30, 31, 32, 58, 62, 92, and 93 were considered. A total of 166 patients were analyzed. HCV genotypes were as follows: G1a (31.9%), G1b (48.2%), G3 (10.2%), and G4 (9.6%). Overall, 69 (41.6%) patients were coinfected with HIV and 46.7% had advanced liver fibrosis (Metavir F3\u2010F4). Sixty (36.1%) patients had at least one RAS at baseline, including M28A\/G\/T (5), Q30X (12), L31I\/F\/M\/V (6), T58P\/S (25), Q\/E62D (1), A92\u2009K (7), and Y93C\/H (15). Overall, 4.8% had two or more RAS, being more frequent in G4 (12.5%) followed by G1b (6.3%) and G1a (1.9%). Of 10 (6%) patients that failed DAA therapy, five had baseline NS5A RAS. No association was found for specific baseline RAS, although changes at position 30 were more frequent in failures than cures (22.2% vs 6.4%, <jats:italic>P<\/jats:italic>\u2009=\u20090.074). Moreover, the presence of two or more RAS at baseline was more frequent in failures (HR: 7.2; <jats:italic>P<\/jats:italic>\u2009=\u20090.029). Upon failure, six patients showed emerging RAS, including Q30C\/H\/R (3), L31M (1), and Y93C\/H (2). Baseline NS5A RAS are frequently seen in DAA\u2010na\u00efve HCV patients. Two or more baseline NS5A RAS were found in nearly 5% and were significantly associated to DAA failure. Therefore, baseline NS5A testing should be considered when HCV treatment is planned with NS5A inhibitors.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24971","type":"journal-article","created":{"date-parts":[[2017,10,9]],"date-time":"2017-10-09T11:52:39Z","timestamp":1507549959000},"page":"532-536","source":"Crossref","is-referenced-by-count":20,"title":["Baseline NS5A resistance associated substitutions may impair DAA response in real\u2010world hepatitis C patients"],"prefix":"10.1002","volume":"90","author":[{"given":"Itz\u00edar","family":"Carrasco","sequence":"first","affiliation":[{"name":"Internal Medicine Department Puerta de Hierro Research Institute and University Hospital Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Arias","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Puerta de Hierro Research Institute and University Hospital Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Ben\u00edtez\u2010Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Puerta de Hierro Research Institute and University Hospital Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gemma","family":"Lled\u00f3","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Puerta de Hierro Research Institute and University Hospital Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Requena","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Puerta de Hierro Research Institute and University Hospital Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Cuesta","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Puerta de Hierro Research Institute and University Hospital Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valent\u00edn","family":"Cuervas\u2010Mons","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Puerta de Hierro Research Institute and University Hospital Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5224-7492","authenticated-orcid":false,"given":"Carmen","family":"de Mendoza","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Puerta de Hierro Research Institute and University Hospital Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.7326\/M16-2575"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2017.01.028"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.08.004"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.13327"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3851\/IMP3123"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.09.011"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.04.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01444-15"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/17425255.2016.1209484"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2017.03.014"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvh.12615"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.06.002"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i40.8910"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.27934"},{"key":"e_1_2_7_16_1","first-page":"1","article-title":"Systematic review and network meta\u2010analysis of randomized controlled trials\u2014comparative effectiveness and safety of DAA for treatment\u2010na\u00efve hepatitis C genotype 1","volume":"95","author":"Zhu G","year":"2016","journal-title":"Medicine (Baltimore)"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.10.013"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)62121-2"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvh.12611"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","DOI":"10.3851\/IMP3061","article-title":"Rate and predictors of treatment failure to all\u2010oral HCV regimens outside clinical trials","author":"Arias A","year":"2017","journal-title":"Antivir 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