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In the present study, the prevalence of herpes simplex virus (HSV), Epstein\u2010Barr virus (EBV), and human cytomegalovirus (HCMV) was investigated in 303 healthy adults in Wuhan, a representative city in Central China. Viral\u2010specific IgG and IgM titers were examined in the serum by chemiluminescent immunoassay, and the existence of viral genomic DNA in blood cells was determined by nested PCR. The overall IgG seroprevalences were 81.5%, 95.4%, and 93.7% for HSV, EBV, and HCMV, while the corresponding IgM seroprevalences were only 6.3%, 2.3%, and 0. The viral genomic DNA of HSV, EBV, and HCMV was identified in the blood samples of 5.9%, 14.2%, and 22.8% of the tested donors, respectively. Significantly, less HSV IgM\u2010positive samples were found in the population over 20 years old than below 20 group; female displayed higher chances for HSV IgG and genome positivity; and occupations such as waiters and medical staffs were shown to be with higher risk for HCMV genome positivity. This study provided useful reference data for the HSV, EBV, and HCMV prevalence in central China, and suggested the potential importance of detecting viral genome to complement serum test data.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.24989","type":"journal-article","created":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T13:26:46Z","timestamp":1509542806000},"page":"571-581","source":"Crossref","is-referenced-by-count":33,"title":["Serologic and viral genome prevalence of HSV, EBV, and HCMV among healthy adults in Wuhan, China"],"prefix":"10.1002","volume":"90","author":[{"given":"Le","family":"Wen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Virology CAS Center for Excellence in Brain Science and Intelligence Technology (CEBSIT) Wuhan Institute of Virology Chinese Academy of Sciences Wuhan China"},{"name":"University of Chinese Academy of Sciences Beijing 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