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The strains were detected and identified in Japan in cases of adenoviral conjunctivitis including epidemic keratoconjunctivitis (EKC). The type was designated as HAdV\u201085 based on the novel combination of penton base (<jats:italic>P<\/jats:italic>\u2009=\u2009HAdV\u201037), hexon (<jats:italic>H<\/jats:italic>\u2009=\u2009HAdV\u201019), and fiber (<jats:italic>F<\/jats:italic>\u2009=\u2009HAdV\u20108). The whole genome sequence determined for HAdV\u201085 was compared against sequences of other types in the same species. The results of the phylogenetic analysis suggested a recombinant origin between HAdV\u201053 and HAdV\u201064, which have been two major causes of adenoviral EKC in Japan over the past decade. During the period between 2008 and 2016 in Kumamoto city, southwest of Japan, 311 cases diagnosed with conjunctivitis were diagnosed as being the consequence of adenoviral infections. Among them, 11 cases were determined to have been caused by HAdV\u201085 since 2015. 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