{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T23:40:42Z","timestamp":1788997242801,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T00:00:00Z","timestamp":1524700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>Human T\u2010cell lymphoma virus (HTLV) has been associated with various disease types. Since the discovery of the virus in 1980, seven subtypes of the virus have been identified. HTLV is widespread and endemic in some regions, such as Japan, Africa, South America, and northeast Iran. This study aimed to identify HTLV\u20101 genotype and also to analyze the nucleotide sequence of the LTR region in three groups, including blood donors, HIV\u20101+ patients, and \u03b2\u2010thalassemia patients. In this cross\u2010sectional study, 2200 samples were collected from blood donors in Tehran (2000 samples), HIV\u20101+ patients (100 samples) and \u03b2\u2010thalassemia patients (100 samples). All samples were screened for anti\u2010HTLV\u2010I&amp;II antibodies by ELISA. Then, genomic DNA was extracted from repeatedly positive samples, and nested PCR was performed for both the TAX and LTR regions. Purified PCR products were sequenced and analyzed, and finally, a phylogenetic tree was constructed using Mega7 software. The prevalence of the anti\u2010HTLV\u2010I&amp;II antibody among blood donors and HIV\u20101+ patients was 1.7% (34\/2000) and 12% (12\/100), respectively. The PCR results confirmed that 0.05% (1\/2000) of blood donors, 5% (5\/100) of HIV\u20101+ patients, and 8% (8\/100) of \u03b2\u2010thalassemia patients were HTLV\u2010I positive. All sequences were matched to HTLV\u20101 subtype a, subgroup A. Our phylogenetic analysis revealed that all sequenced samples belong to the endemic clusters of Iran. HTLV\u20101 genotypes in all samples were similar in three groups and were derived from the strains, which had been previously reported from Iran (AF00300\/Mashhad and KT190712.1\/Sabzevar).<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.25192","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T01:11:53Z","timestamp":1523927513000},"page":"1398-1405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Phylogenetic analysis of HTLV\u20101 in Iranian blood donors, HIV\u20101 positive patients and patients with beta thalassemia"],"prefix":"10.1002","volume":"90","author":[{"given":"Leila","family":"Pirayeshfard","sequence":"first","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran 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