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Influenza A (H3N2) virus\u2013infected male C57Bl6 mice were treated intravenously with either AT10_002 or a control 2 days postinfection. Seven days later, both groups were infected with <jats:italic>Streptococcus pneumoniae<\/jats:italic> and killed 18\u2009hours later. Mice receiving AT10_002 showed less loss of bodyweight compared with controls (+1% vs \u221212%, <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001), lower viral loads in bronchoalveolar lavage fluids (BALFs) (7 vs 194 RNA copies per \u00b5L; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001), and reduced bacterial outgrowth in lung homogenates (3.3\u2009\u00d7\u200910<jats:sup>1<\/jats:sup> vs 2.5\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> colony\u2010forming units per mg; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001). The treatment group showed lower pulmonary wet weights, lower cell counts, and lower protein levels in BALF compared with controls. Treatment with AT10_002 was associated with lower levels of tumor necrosis factor\u2010\u03b1, interleukin (IL)\u20106, cytokine\u2010induced neutrophil chemoattractant (KC), and interferon\u2010\u03b3 in BALF and lower IL\u20106 and KC in lung homogenates. Treatment with anti\u2010influenza antibody AT10_002 is associated with reduced weight loss, viral load, bacterial outgrowth, and lung injury in a murine model of secondary pneumococcal pneumonia following influenza infection.<\/jats:p>","DOI":"10.1002\/jmv.25212","type":"journal-article","created":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T14:21:30Z","timestamp":1525270890000},"page":"1431-1437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Treatment with broadly neutralizing influenza antibodies reduces severity of secondary pneumococcal pneumonia in mice"],"prefix":"10.1002","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6984-6683","authenticated-orcid":false,"given":"Frank","family":"van Someren Gr\u00e9ve","sequence":"first","affiliation":[{"name":"Department of Intensive Care Academic Medical Center  Amsterdam The Netherlands"},{"name":"Laboratory of Experimental Intensive Care and Anesthesiology (LEICA) Academic Medical Center  Amsterdam The Netherlands"},{"name":"Department of Medical Microbiology Academic Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koenraad F.","family":"van der Sluijs","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Intensive Care and Anesthesiology (LEICA) Academic Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita M.","family":"Tuip","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Intensive Care and Anesthesiology (LEICA) Academic Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus J.","family":"Schultz","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Academic Medical Center  Amsterdam The Netherlands"},{"name":"Laboratory of Experimental Intensive Care and Anesthesiology (LEICA) Academic Medical Center  Amsterdam The Netherlands"},{"name":"Faculty of Tropical Medicine, Mahidol Oxford Research Unit (MORU) Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menno D.","family":"de Jong","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Academic Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole P.","family":"Juffermans","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Academic Medical Center  Amsterdam The Netherlands"},{"name":"Laboratory of Experimental Intensive Care and Anesthesiology (LEICA) Academic Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,12]]},"reference":[{"key":"e_1_2_7_1_2_1","unstructured":"WHO Influenza Fact sheet No. 211.2014.http:\/\/www.who.int\/mediacentre\/factsheets\/fs211\/en\/(accessed 9 Nov 2016)."},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2012.01.003"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2010.0049"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc8893"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000347"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00298-15"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-6-58"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201101-0110OC"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-010-2078-z"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.3.4.397"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00041-5"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00070-7"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-017-2905-8"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis574"},{"key":"e_1_2_7_1_16_1","unstructured":"Centers for Disease Control and Prevention (CDC). 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