{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:53:44Z","timestamp":1790834024776,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,5,25]],"date-time":"2018-05-25T00:00:00Z","timestamp":1527206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI050611"],"award-info":[{"award-number":["R01 AI050611"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI074667"],"award-info":[{"award-number":["R01 AI074667"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:p>Hepatitis E virus (HEV), a member of the family <jats:italic>Hepeviridae<\/jats:italic>, causes both acute and chronic viral hepatitis. We have previously demonstrated that the stem\u2010loop structure in the junction region (JR) of HEV genome plays a critical role in HEV replication. However, the function of the sequence bordering the JR, including the 3\u2032 terminus of open reading frame (ORF1), in HEV replication is unknown. In this study, a panel of HEV Renilla luciferase (Rluc) replicons containing various deletions at 5\u2032 or 3\u2032 termini of the JR was constructed to determine the effect of the deletions on HEV replication in Huh7 human liver cells. We showed that even a single nucleotide deletion at the 5\u2032 terminus of the JR abolished HEV replication, whereas deletions at the 3\u2032 terminus of the JR also decreased virus replication efficiency. Furthermore, we also constructed firefly luciferase\u00a0and Rluc dual\u2010reporter HEV replicons containing the 3\u2032 terminal ORF1 of various lengths and the JR inserted upstream of the Rluc reporter. A higher level of HEV replication was observed in cells transfected with replicons containing the 3\u2032 terminal ORF1 than that of the JR only replicon. We also showed that the ORF3 noncoding sequence along with the JR promoted a higher level of translation activity than that promoted by JR and the ORF2 noncoding sequence.<\/jats:p>","DOI":"10.1002\/jmv.25215","type":"journal-article","created":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T14:05:46Z","timestamp":1525269946000},"page":"1524-1531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Roles of the genomic sequence surrounding the stem\u2010loop structure in the junction region including the 3\u2032 terminus of open reading frame 1 in hepatitis E virus replication"],"prefix":"10.1002","volume":"90","author":[{"given":"Dianjun","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Virginia\u2010Maryland College of Veterinary Medicine Virginia Polytechnic Institute and State University  Blacksburg 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