{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T06:34:22Z","timestamp":1792132462227,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,8,16]],"date-time":"2018-08-16T00:00:00Z","timestamp":1534377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81501835"],"award-info":[{"award-number":["81501835"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science and Technology Major Project of the Fujian Province","award":["3502Z20171001-20170302"],"award-info":[{"award-number":["3502Z20171001-20170302"]}]},{"name":"Natural Science Foundation of the Fujian Province","award":["2017J05136"],"award-info":[{"award-number":["2017J05136"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>Zika virus (ZIKV), dengue virus (DENV), chikungunya virus (CHIKV) and yellow fever virus (YFV) share the same mosquito vectors and have similar clinical manifestations early stage of infection. Therefore, simultaneously differentiating these viruses from each other is necessary. We developed a multiplex real\u2010time reverse\u2010transcriptase polymerase chain reaction (RT\u2010PCR) assay for the differentiation of these four viruses in a single tube. The linear range was established by regression analysis, and the <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> value for each virus\u00a0was \u22650.98, and the 95% lower limit of detection\u00a0for each virus was as follows (copies\/reaction): ZIKV\u2010Asian, 9; ZIKV\u2010Africa, 15; CHIKV, 11; DENV\u20101, 19; DENV\u20102, 13; DENV\u20103, 24; DENV\u20104, 36; and YFV, 17. Meanwhile, our multiplex real\u2010time RT\u2010PCR has a good consistency with the commercial singleplex assay. In summary, the developed assay can be effectively used for the diagnosis of ZIKV, DENV, CHIKV, and YFV infections.<\/jats:p>","DOI":"10.1002\/jmv.25253","type":"journal-article","created":{"date-parts":[[2018,7,6]],"date-time":"2018-07-06T17:44:11Z","timestamp":1530899051000},"page":"1681-1686","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Development of multiplex real\u2010time reverse\u2010transcriptase polymerase chain reaction assay for simultaneous detection of Zika, dengue, yellow fever, and chikungunya viruses in a single tube"],"prefix":"10.1002","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3209-1341","authenticated-orcid":false,"given":"Wenhui","family":"Wu","sequence":"first","affiliation":[{"name":"National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University Xiamen China"},{"name":"State Key Laboratory of Molecular 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