{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T05:11:41Z","timestamp":1780981901499,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T00:00:00Z","timestamp":1541116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005270","name":"Fujian Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["2015J01385"],"award-info":[{"award-number":["2015J01385"]}],"id":[{"id":"10.13039\/501100005270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005270","name":"Fujian Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["2016Y9020"],"award-info":[{"award-number":["2016Y9020"]}],"id":[{"id":"10.13039\/501100005270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672101"],"award-info":[{"award-number":["81672101"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702073"],"award-info":[{"award-number":["81702073"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572067"],"award-info":[{"award-number":["81572067"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>The detection of hepatitis B virus (HBV) DNA plays a critical role in determining the level of viral replication in HBV\u2010infected patients. However, how to select appropriate HBV DNA detection method, low\u2010sensitivity (ls) and hypersensitivity (hs) remains unclear. In this study, hepatitis B surface antigen\u00a0(HBsAg), hepatitis B e\u2010antigen (HBeAg), alanine transaminase (ALT), aspartate transaminase (AST), and hs HBV DNA titers in serum of 5611 cases with suspected HBV infection were reviewed. Besides, the dynamic changes of HBV DNA and HBsAg in 85 chronic hepatitis B (CHB) patients receiving peginterferon \u03b1 (PegIFN\u03b1) or entecavir (ETV) were observed. The results showed the positive rate of HBV DNA was 32.8%, of which low viral load (20 to 500\u2009IU\/mL) accounted for 51.8%. In the 5611 cases, when the HBsAg was less than 1000\u2009IU\/mL, the proportion of low viral load was 76.3%. Moreover, in patients receiving antiviral treatment, when HBsAg was less than 2000\u2009IU\/mL (PegIFN\u03b1) or HBsAg was less than 3500\u2009IU\/mL (ETV), the proportion of patients with low viral load was 79.5% or 78.0%, respectively. We developed a strategy of serum HBV DNA detection in HBV\u2010infected patients. When HBsAg was negative, HBV DNA detection should be unnecessary. When HBsAg was 0.05 to 1000\u2009IU\/mL, hs HBV DNA should be detected in patients with abnormal level of ALT, AST, or HBeAg. While HBsAg was greater than or equal to 1000\u2009IU\/mL, ls HBV DNA was recommended. Moreover, the cutoff value of HBsAg increased during antiviral therapy of CHB patients. In conclusion, hs HBV DNA is of great value in HBV\u2010infected patients with low viral load. HBV DNA detection methods should be selected reasonably according to the levels of HBsAg, HBeAg, ALT, and AST.<\/jats:p>","DOI":"10.1002\/jmv.25271","type":"journal-article","created":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T17:19:14Z","timestamp":1531761554000},"page":"249-257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Application strategies of serum HBV DNA detection in HBV infection patients: A retrospective study of 5611 specimens"],"prefix":"10.1002","volume":"91","author":[{"given":"Can","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine The First Af\ufb01liated Hospital of Fujian Medical University Fuzhou China"},{"name":"Gene Diagnostic Laboratory The First Af\ufb01liated Hospital of Fujian Medical University Fuzhou 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