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Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the two major causative agents. The objective of this study was to determine the optimal time and method of HFMD detection, explore the seroconversion of IgM and IgG antibodies, and examine the response of neutralizing antibody (NtAb) to EV71 or CVA16. Between January 2016 and December 2017, a total of 460 patients, diagnosed with HFMD based on clinical symptoms and hospitalized in the First Hospital of Jilin University, were recruited for the study. At approximately 72\u2009hours post illness onset, we observed that the positive rate of both IgM and real\u2010time polymerase chain reaction detection of EV71 or CVA16 was the highest, this could be considered as the optimal detection time for clinical diagnosis. During the initial 0 \u201096\u2009hours, the relative highest IgM and the relative lowest IgG antibody levels were observed. The NtAb titers to EV71 and CVA16 also gradually increased with time, showing a positive correlation with age, and being the predominant factor during the hospitalized days. Thus, our study provides important information for the clinical diagnosis and treatment of HFMD.<\/jats:p>","DOI":"10.1002\/jmv.25282","type":"journal-article","created":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T20:10:46Z","timestamp":1534536646000},"page":"201-207","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Understanding the epidemiological characteristics of EV71 and CVA16 infection to aid the diagnosis and treatment of hand, foot, and mouth disease"],"prefix":"10.1002","volume":"91","author":[{"given":"Bin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases The First Hospital of Jilin University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingliang","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Virology and AIDS Research, The First Hospital of Jilin University Changchun 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