{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T07:29:32Z","timestamp":1773646172841,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,31]],"date-time":"2018-10-31T00:00:00Z","timestamp":1540944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","award":["DPG5980005"],"award-info":[{"award-number":["DPG5980005"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p>The Epstein\u2010Barr virus (EBV) is one of the infectious agents found in stomach tissue. Recently, EBV\u2010associated gastric carcinoma (EBVaGC) was classified as a new subtype of gastric carcinoma. To date, there is a lack of knowledge about the distribution and prevalence of EBV infection in both the normal stomach and various gastric lesions, including EBVaGC, in the Thai population. In this study, we detected EBV in the normal stomach (NS; n\u2009=\u200919), chronic gastritis (CG; n\u2009=\u200936), intestinal metaplasia (IM; n\u2009=\u200940), gastric dysplasia (GD; n\u2009=\u200915), and gastric adenocarcinoma (GC; n\u2009=\u200933) by polymerase chain reaction (PCR) amplification of the latent membrane protein (<jats:italic>LMP1<\/jats:italic>) gene of EBV. EBV\u2010PCR amplification was positive in 42.1%, 36.1%, 22.5%, 13.3%, and 33.3% of NS, CG, IM, GD, and GC, respectively. For further clarification in EBVaGC, we performed EBV\u2010encoded small RNA in situ hybridization (EBER\u2010ISH) in PCR\u2010positive cases of GD and GC. Four GC cases were EBER\u2010ISH positive (12.1%), while both GD cases were EBER\u2010ISH negative. In addition, we determined the distribution of the EBV strain (type A or B) based on <jats:italic>EBNA3C<\/jats:italic> sequence and EBV variants based on <jats:italic>LMP1<\/jats:italic> variation (wild\u2010type and 30\u2010bp deletion variants; wt\u2010LMP1 or del\u2010LMP1). The results showed that type A and wt\u2010LMP1 were the most prevalent in all lesions. In conclusion, EBV is common in both the NS and gastric lesions, and the frequency of EBVaGC was 12.1% in Thai patients.<\/jats:p>","DOI":"10.1002\/jmv.25318","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T10:36:31Z","timestamp":1537353391000},"page":"444-449","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Distribution of the Epstein\u2010Barr virus in the normal stomach and gastric lesions in Thai population"],"prefix":"10.1002","volume":"91","author":[{"given":"Supitcha","family":"Wanvimonsuk","sequence":"first","affiliation":[{"name":"Department of Biochemistry Faculty of Science, Chulalongkorn University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pichaya","family":"Thitiwanichpiwong","sequence":"additional","affiliation":[{"name":"Research and International Relations Division HRH Princess Chulabhorn College of Medical Science Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somboon","family":"Keelawat","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Medicine, Chulalongkorn University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Apiwat","family":"Mutirangura","sequence":"additional","affiliation":[{"name":"Department of Anatomy Faculty of Medicine, Center of Excellence in Molecular Genetics of Cancer and Human Diseases Chulalongkorn University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0616-6039","authenticated-orcid":false,"given":"Nakarin","family":"Kitkumthorn","sequence":"additional","affiliation":[{"name":"Department of Oral Biology Faculty of Dentistry, Mahidol University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,31]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2009.06188.x"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gtc.2013.01.006"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-3301"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-0670-3"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13480"},{"issue":"3","key":"e_1_2_6_1_7_1","first-page":"377","article-title":"Lymphoepithelial carcinoma of the stomach with Epstein\u2010Barr virus demonstrated by polymerase chain reaction","volume":"3","author":"Burke AP","year":"1990","journal-title":"Mod Pathol"},{"issue":"3","key":"e_1_2_6_1_8_1","first-page":"469","article-title":"Association of Epstein\u2010Barr virus with undifferentiated gastric carcinomas with intense lymphoid infiltration. 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