{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T11:34:09Z","timestamp":1761824049658,"version":"3.37.3"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,12,17]],"date-time":"2018-12-17T00:00:00Z","timestamp":1545004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E\u201026\/202.930\/2016"],"award-info":[{"award-number":["E\u201026\/202.930\/2016"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["402571\/2011\u20108"],"award-info":[{"award-number":["402571\/2011\u20108"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>The hyperendemicity and co\u2010circulation of different dengue serotypes in Brazil have increased the number of severe dengue cases and the rate of hospitalization for dengue. Virological and individual factors are associated with the complexity of the disease. Antigenemia levels of nonstructural glycoprotein\u20101 (NS1) have been associated with severe dengue. Aiming to identify a severity marker during the acute phase (days 0 to 5 of disease), the association of NS1 antigenemia with clinical presentation, sex, age range, immune response, number of days of disease, and serotype RNA levels was evaluated in serum samples of patients from the state of Rio de Janeiro clinically classified as having dengue without warning signs (DWWS) or dengue with warning signs\/severe dengue (DWWS\/SD). The immune response was classified by in\u2010house enzyme\u2010linked immunosorbent assay, antigenemia was determined by quantification of NS1, and viremia was quantified by real\u2010time PCR. Of the total number of patients, 36.6% (74 of 202) presented warning signs\/severe dengue and 72.3% (146 of 202) were classified with primary infection. DENV\u20102 presented an association between clinical presentation and antigenemia (<jats:italic>P<\/jats:italic>\u2009=\u20090.02). DENV\u20103 had higher levels of NS1 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). This study has shown that the infecting serotype influences circulating NS1 levels in the host, as well as NS1 antigenemia may vary as to the clinical presentation of the patient infected with DENV\u20102. However, the criterion used to screen patients for clinical presentation, in DWWS and DWWS\/SD patients, was not a good marker for dengue severity in our study.<\/jats:p>","DOI":"10.1002\/jmv.25356","type":"journal-article","created":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T06:31:24Z","timestamp":1541745084000},"page":"555-563","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Dynamics of nonstructural glycoprotein\u20101 in dengue patients presenting with different clinical manifestations from 1986 to 2012 in Rio de Janeiro, Brazil"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3135-8008","authenticated-orcid":false,"given":"Bianca De Santis","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Institute\/FIOCRUZ  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco Aur\u00e9lio Pereira","family":"Horta","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Institute\/FIOCRUZ  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro Hernan Cabello","family":"Acero","sequence":"additional","affiliation":[{"name":"Laboratory of Human Genetics Oswaldo Cruz Institute\/FIOCRUZ  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosany","family":"Bochner","sequence":"additional","affiliation":[{"name":"Laboratory of Scientific and Technological Information in Health Institute of Communication and Scientific and Technological Information in Health\/ FIOCRUZ  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique da Rocha","family":"Queiroz Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Immunology Oswaldo Cruz Institute\/FIOCRUZ  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliane Saraiva","family":"de Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Institute\/FIOCRUZ  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone Alves","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Institute\/FIOCRUZ  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita Maria Ribeiro","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Institute\/FIOCRUZ  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Maria Bispo","family":"de Filippis","sequence":"additional","affiliation":[{"name":"Flavivirus Laboratory Oswaldo Cruz Institute\/FIOCRUZ  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,17]]},"reference":[{"key":"e_1_2_7_1_2_1","unstructured":"2009 WHO Geneva Switzerland World Health Organization\/ Tropical Diseases Research Dengue guidelines for diagnosis treatment prevention and control"},{"key":"e_1_2_7_1_3_1","first-page":"pp. 991","volume-title":"Fields Virology","author":"Lindenbach BD","year":"2001"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(90)90075-Q"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050127"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.10-0138"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.02.376-381.2002"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.02.003"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113634"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1053-1057.2000"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa3787"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir014"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.12-0023"},{"issue":"1","key":"e_1_2_7_1_14_1","first-page":"53","article-title":"Surto de dengue em Boa Vista, Roraima: Nota Pr\u00e9via","volume":"25","author":"Osanai CH","year":"1983","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761986000200019"},{"issue":"3","key":"e_1_2_7_1_16_1","article-title":"Health surveillance","volume":"46","author":"Brazil. 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