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While previous work shows that LMP2A mimics B\u2010cell receptor (BCR) signaling to promote the survival of HRS cells, the ability of LMP2A to establish and maintain the tumor microenvironment through the production of chemokines remains unknown. Since BCR signaling induces the production of the chemokine macrophage inflammatory protein\u20101\u03b1 (MIP\u20101\u03b1), and since LMP2A is a BCR mimic, we hypothesized that LMP2A increases MIP\u20101\u03b1 levels. A comparison of multiple LMP2A\u2010negative and \u2010positive cell lines demonstrates that LMP2A increases MIP\u20101\u03b1. Additionally, LMP2A\u2010mutant cell lines and pharmacologic inhibitors indicate that LMP2A activates a Syk\/PI3K\/NF\u2010\u03baB pathway to enhance MIP\u20101\u03b1. Finally, based on the finding that an NF\u2010\u03baB inhibitor decreased MIP\u20101\u03b1 RNA\/protein in LMP2A\u2010positive cells, we are the first to demonstrate that LMP2A increases the nuclear localization of the NF\u2010\u03baB p65 subunit using DNA\u2010binding assays and confocal microscopy in human B cells. These findings not only have implications for the treatment of HL, but also other LMP2A\u2010expressing B\u2010cell tumors that overexpress NF\u2010\u03baB.<\/jats:p>","DOI":"10.1002\/jmv.25381","type":"journal-article","created":{"date-parts":[[2019,1,4]],"date-time":"2019-01-04T18:43:12Z","timestamp":1546627392000},"page":"845-855","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Epstein\u2010Barr virus LMP2A utilizes Syk and PI3K to activate NF\u2010\u03baB in B\u2010cell lymphomas to increase MIP\u20101\u03b1 production"],"prefix":"10.1002","volume":"91","author":[{"given":"Ryan","family":"Incrocci","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology College of Graduate Studies, Midwestern University Downers Grove Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"McAloon","sequence":"additional","affiliation":[{"name":"Chicago College of 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