{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:07:48Z","timestamp":1782976068923,"version":"3.54.5"},"reference-count":59,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T00:00:00Z","timestamp":1548028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:p>Norovirus (NoV) is a major cause of nonbacterial acute gastroenteritis (AGE) outbreaks worldwide, with infections reported in semiclosed environments, particularly in hospitals and nursing homes. Astrovirus (HAstV) is prevalent worldwide, especially in developing countries. We aimed to determine the prevalence, spatial distribution, and genetic diversity of NoV and HAstV in children under 5 years of age in Rio Branco city, Acre State, Amazon Region, Brazil. Stool samples from children with (n\u2009=\u2009240) and without (n\u2009=\u2009248) AGE were collected from January to December 2012 from seven neighborhoods. The overall NoV prevalence was 12.3% (60 of 488); representing 15.8% (38 of 240) of the symptomatic samples and 8.9% (22 of 248) of the controls. HAstVs infection was observed in 4.7% (23 of 488) of the samples tested, 6.2% (15 of 240) of AGE cases, and 2.4% (6 of 248) of the controls (plus two without information about feces consistency). Infections were found in all age groups with higher frequency in children less than two years of age, for both viruses. NoV was detected in all neighborhoods, with a higher concentration in the fourth (30%; 18 of 60). NoV nucleotide sequencing performed in 86.7% (52 of 60) of the positive samples showed the circulation of the strains GII.4 (57.7%; 30 of 52), GIIPe\/GII.4 (19.2%; 10 of 52), GII.7, GII.Pg\/GII.1, and GII.Pc (3.8%; 2 of 52 for each), GII.6 and GII.Pg (1.9%; 1 of 52 for each), and GI.3 (7.7%; 4 of 52). Three GII.4 variants were detected: Den Haag_2006b (n\u2009=\u20091), New Orleans_2009 (n\u2009=\u20091), and Sydney_2012 (n\u2009=\u200914). HAstV types HAstV\u20101a (81.8%; 9 of 11) and HAstV\u20102c (18.2%; 2 of 11) were observed in the 47.8% (11 of 23) of characterized samples. This is the first data obtained in Acre State regarding the prevalence of these viruses and provides epidemiological and molecular information for a better understanding of their role among children with and without AGE.<\/jats:p>","DOI":"10.1002\/jmv.25395","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T16:42:06Z","timestamp":1547052126000},"page":"997-1007","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Epidemiological and molecular investigation of norovirus and astrovirus infections in Rio Branco, Acre, Northern Brazil: A retrospective study"],"prefix":"10.1002","volume":"91","author":[{"given":"Evandro Leite Rodrigues","family":"Bitencurt","sequence":"first","affiliation":[{"name":"Postgraduate Program in Parasitic Biology in the Amazon, University of the State of Par\u00e1 Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6491-6590","authenticated-orcid":false,"given":"Jones Anderson Monteiro","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tallyta Barros","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato da Silva","family":"Bandeira","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darleise","family":"de Souza Oliveira","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Jos\u00e9","family":"de Paula Souza e Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana","family":"da Silva Soares","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1241-8258","authenticated-orcid":false,"given":"Joana D'Arc Pereira","family":"Macarenhas","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dielle Monteiro","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita S.U.","family":"Silva","sequence":"additional","affiliation":[{"name":"Municipality Secretary of Health of Rio Branco  Acre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edvaldo Carlos Brito","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f4nica Cristina","family":"de Moraes Silva","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Damascena","family":"da Silva","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1242-0523","authenticated-orcid":false,"given":"Yvone Benchimol","family":"Gabbay","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute; Health Surveillance Secretariat, Brazilian Ministry of Health Ananindeua Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,21]]},"reference":[{"key":"e_1_2_6_1_2_1","unstructured":"WHO. 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