{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T13:22:56Z","timestamp":1788009776834,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,1,28]],"date-time":"2019-01-28T00:00:00Z","timestamp":1548633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["Grant\u2010in\u2010Aid for Scientific Research (16809509)"],"award-info":[{"award-number":["Grant\u2010in\u2010Aid for Scientific Research (16809509)"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare and evaluate the sensitivity of a newly developed silver\u2010amplified immunochromatography (SAI) kit with various immunochromatography (IC) kits for adenoviruses based on the detection limit (copies\/test).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis\u2010associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). The detection limits were presented as numerical values as determined by real\u2010time polymerase chain reaction (PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The detection limit of the SAI kit for the adenovirus types ranged from 1.0\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\u20135.0\u2009\u00d7\u200910\n<jats:sup>4<\/jats:sup>\u2009copies\/test (geometric mean, 4.7\u2009\u00d7\u200910\n<jats:sup>3<\/jats:sup>). SAI had a 10\u2013250\u2010fold lower detection limit than the four IC kits for all adenoviruses studied. There were also differences in detection limits among the adenovirus types for each kit.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The detection limit of the SAI kit was drastically reduced because the silver\u2010amplification reaction increased the color development sensitivity. The results revealed the high sensitivity of SAI for detecting adenoviruses and suggested its usefulness for conjunctivitis examination.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.25404","type":"journal-article","created":{"date-parts":[[2019,1,19]],"date-time":"2019-01-19T20:02:50Z","timestamp":1547928170000},"page":"1030-1035","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of a silver\u2010amplified immunochromatography kit for adenoviral conjunctivitis"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4861-4349","authenticated-orcid":false,"given":"Tsuguto","family":"Fujimoto","sequence":"first","affiliation":[{"name":"Infectious Disease Surveillance Center National Institute of Infectious Diseases Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomu","family":"Hanaoka","sequence":"additional","affiliation":[{"name":"Infectious 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