{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T02:04:06Z","timestamp":1779242646880,"version":"3.51.4"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T00:00:00Z","timestamp":1549324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Maharashtra State (MS), India, launched measles surveillance in the year 2013. From 2014 to 2017, specimens from the patients presenting with fever and skin rashes were received at the National Institute of Virology, Pune. From 36 districts of the MS, 2795 cases (1428 males and 1367 females) were referred for the laboratory diagnosis of measles and rubella using immunoglobulin M enzyme immunoassay and\/or RT\u2010PCRs. The majority of the cases (93.3%, n\u2009=\u20092609) were under 15 years of age. About 17.7% (494) cases had a history of measles immunization (one dose) during their childhood. Virus isolation was attempted from 107 throat swabs and 84 urine samples obtained from 191 cases using Vero hSLAM cells. The results confirmed measles in 1756 and rubella\u00a0in 282 fever with rash cases by serological and molecular tools. Precisely, 170 of 382 and 35 of 149 specimens were positive for measles and rubella RT\u2010PCRs, respectively. Sequencing of the representative PCR products showed the circulation of measles virus genotypes D4 (n\u2009=\u200926) and D8 (n\u2009=\u2009107), and rubella virus genotype 2B (n\u2009=\u20091). Twenty\u2010three measles viruses were isolated and genotyped, of which, 6 were D4 and 17 were D8 genotypes. Amongst the measles\u2010immunized individuals, 51.2% (253\/494) had laboratory\u2010confirmed measles. Overall, 72.9% fever with skin rash cases (n\u2009=\u20092038) from the MS was laboratory confirmed for either measles or rubella. The contribution of measles was higher than rubella in the fever with rash cases. As expected, more fever with rash cases were documented in children compared with adults and highlighted the need to increase measles\u2010rubella immunization coverage.<\/jats:p>","DOI":"10.1002\/jmv.25407","type":"journal-article","created":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T04:36:10Z","timestamp":1548390970000},"page":"972-978","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Laboratory\u2010based investigation of fever with rash cases in the Maharashtra State \u2010 India, 2014 to 2017"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6157-9090","authenticated-orcid":false,"given":"Sunil R.","family":"Vaidya","sequence":"first","affiliation":[{"name":"Measles Group ICMR\u2010National Institute of Virology Pune Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhukar B.","family":"Kamble","sequence":"additional","affiliation":[{"name":"Measles Group ICMR\u2010National Institute of Virology Pune Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelakshi S.","family":"Kumbhar","sequence":"additional","affiliation":[{"name":"Measles Group ICMR\u2010National Institute of Virology Pune Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,5]]},"reference":[{"key":"e_1_2_6_1_2_1","first-page":"73","article-title":"Measles virus nomenclature update: 2012","volume":"87","author":"World Health Organization","year":"2012","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10228"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-007-0172-2"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir150"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24702"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13312-011-0066-1"},{"key":"e_1_2_6_1_8_1","unstructured":"World Health OrganizationSouth\u2010East Asia Regional Office. 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