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Early diagnosis is crucial to improve patient management and to reduce the risk of complications. A glass\u2010fiber laser\u2010cut microfluidic device (paper\u2010based analytical device [PAD]) was designed and evaluated in a proof of principle context, for the analysis of 30\u2009\u00b5L of patient serum. Biological raw materials used for the functionalization of the PAD were first screened by MAC\u2010ELISA (IgM capture enzyme\u2010linked immunosorbent assay) for CHIKV Immunoglobulin M (IgM) capture and then evaluated on the PAD using various human samples. Compared with viral lysate traditionally used for chikungunya (CHIK) serology, CHIKV pseudo\u2010particles (PPs) have proven to be powerful antigens for specific IgM capture. The PAD was able to detect CHIKV IgM in human sera in less than 10 minutes. Results obtained in patient sera showed a sensitivity of 70.6% and a specificity of around 98%. The PAD showed few cross\u2010reactions with other tropical viral diseases. The PAD could help health workers in the early diagnosis of tropical diseases such as CHIK, which require specific management protocols in at\u2010risk populations.<\/jats:p>","DOI":"10.1002\/jmv.25420","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T09:39:19Z","timestamp":1549618759000},"page":"899-910","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Detection of chikungunya virus\u2010specific IgM on laser\u2010cut paper\u2010based device using pseudo\u2010particles as capture antigen"],"prefix":"10.1002","volume":"91","author":[{"given":"Gerald","family":"Theillet","sequence":"first","affiliation":[{"name":"bioM\u00e9rieux, Innovations New Immuno\u2010Concepts department, Chemin de l'Orme Marcy\u2010l'Etoile France"},{"name":"Unit\u00e9 des Virus Emergents (UVE: Aix\u2010Marseille Univ, IRD 190, Inserm 1207, IHU M\u00e9diterran\u00e9e Infection) Marseille 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