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This study aimed to investigate their epidemiological profile in Portuguese elderly living in long\u2010term care facilities and to assess the host genetic factors mediating infection susceptibility. From November 2013 to June 2015, 636 faecal specimens from 169 elderly, mainly asymptomatic, living in nursing homes in Greater Lisbon and Faro district, Portugal, were collected. NoV and RVA were detected by real\u2010time polymerase chain reaction and NoV genotyped by phylogenetic analysis. NoV detection rate was 7.1% (12 of 169). Three GI.3 and one GII.6 strains were genotyped. RVA detection rate was 3.6% (6 of 169), exclusively in asymptomatic individuals. Host genetic factors associated with infection susceptibility were described on 250 samples by saliva\u2010based enzyme\u2010linked immunosorbent assays. The Lewis\u2010negative phenotype was 8.8% (22 of 250) and the rate of nonsecretors was 16.8% (42 of 250). Association to NoV and RVA infection was performed in the subgroup of individuals (<jats:italic>n<\/jats:italic>\u2009=\u2009147) who delivered both faecal and saliva samples. The majority of NoV\u2010 and RVA\u2010positive individuals (90.9% and 83.3%, respectively) were secretor\u2010positive, with Lewis B phenotype. In a subset of individuals, \n<jats:italic>FUT2<\/jats:italic> and \n<jats:italic>FUT3<\/jats:italic> genes were genotyped to assess mutations and validate the secretor and Lewis phenotypes. All sequenced nonsecretors were homozygous for \n<jats:italic>FUT2<\/jats:italic> nonsense mutation G428A. In this study, low detection rates of NoV and RVA infections were found during two winter seasons. However, even in the absence of any outbreak, the importance of finding these infections in a nonepidemic situation in long\u2010term care facilities may have important implications for infection control.<\/jats:p>","DOI":"10.1002\/jmv.25425","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T15:07:18Z","timestamp":1549638438000},"page":"1014-1021","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular epidemiology and host genetics of norovirus and rotavirus infections in Portuguese elderly living in aged care homes"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6384-1737","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Piedade","sequence":"first","affiliation":[{"name":"Unidade de Microbiologia M\u00e9dica Global Health and Tropical Medicine (GHTM), Instituto de Higiene e Medicina Tropical (IHMT), Universidade NOVA de Lisboa 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