{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:12:47Z","timestamp":1791223967429,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T00:00:00Z","timestamp":1552262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["444494\/2014\u20106"],"award-info":[{"award-number":["444494\/2014\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002222","name":"The World Academy of Sciences","doi-asserted-by":"publisher","award":["190054\/2012\u20103"],"award-info":[{"award-number":["190054\/2012\u20103"]}],"id":[{"id":"10.13039\/501100002222","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human adenovirus (HAdV\u20102) is considered a common agent of respiratory tract infection in the human, especially in children. Virus infection is believed to modify host cell expression necessary for its replication and therefore cell proteome can reflect the changes of specific cellular pathways during infection. This study aims to identify differentially expressed proteins of A549 cells in response to HAdV\u20102 infection using a label\u2010free liquid chromatography\u2010high\u2010resolution tandem mass spectrometry strategy (LC\u2010MS\/MS) at 24 and 48 hpi. A total of 248 and 216 proteins were deregulated by 1.35\u2010fold at 24 and 48 hpi, respectively. Among them, 155 were upregulated at 24 hpi and 86 at 48 hpi, whereas 93 and 130 were downregulated at 24 and 48 hpi, respectively. The identified proteins were involved in different pathways as energy, transcription, protein synthesis, cytoskeleton, rescue and defense, cell cycle, DNA processing, transportation, and metabolism. Glycolytic pathway and histone deregulated proteins were further confirmed by chemical testing and immunofluorescence, respectively. The results suggest that the identified proteins influenced HAdV\u20102 infection in the context of viral replication and propagation. This study complement proteomic data obtained from previous studies and reinforce the understanding of the relationship between HAdV and host cell.<\/jats:p>","DOI":"10.1002\/jmv.25439","type":"journal-article","created":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T14:18:46Z","timestamp":1550845126000},"page":"1239-1249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Quantitative proteomic analysis of A549 cells infected with human adenovirus type 2"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1544-9869","authenticated-orcid":false,"given":"Kareem R.","family":"Badr","sequence":"first","affiliation":[{"name":"Department of Microbiology, Human Virology Laboratory Institute of Tropical Pathology and Public Health, Federal University of Goi\u00e1s Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana A.","family":"Parente\u2010Rocha","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology Laboratory Institute of Biological Sciences, Federal University of Goi\u00e1s Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilian C.","family":"Baeza","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology Laboratory Institute of Biological Sciences, Federal University of Goi\u00e1s Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiola S.","family":"Ficcadori","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Human Virology Laboratory Institute of Tropical Pathology and Public Health, Federal University of Goi\u00e1s Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menira","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Human Virology Laboratory Institute of Tropical Pathology and Public Health, Federal University of Goi\u00e1s Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia M.","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology Laboratory Institute of Biological Sciences, Federal University of Goi\u00e1s Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carla P.","family":"Guissoni","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Human Virology Laboratory Institute of Tropical Pathology and Public Health, Federal University of Goi\u00e1s Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e2mera N.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Human Virology Laboratory Institute of Tropical Pathology and Public Health, Federal University of Goi\u00e1s Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0946-6168","authenticated-orcid":false,"given":"Divina D.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Human Virology Laboratory Institute of Tropical Pathology and Public Health, Federal University of Goi\u00e1s Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,11]]},"reference":[{"key":"e_1_2_8_1_2_1","first-page":"125","volume-title":"Virus taxonomy: classification and nomenclature of viruses. 9th report of the International Committee on Taxonomy of Viruses","author":"Harrach B","year":"2011"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25041"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.1999.0656"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00156-11"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19497-0"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1076"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.2.190"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.926401"},{"key":"e_1_2_8_1_10_1","first-page":"97","article-title":"The multifunctional role of E1A in the transcriptional regulation of CREB\/CBP\u2010dependent target genes","volume":"272","author":"Brockmann D","year":"2003","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_8_1_11_1","first-page":"233","article-title":"Structural insights into recognition of adenovirus by immunologic and serum factors","volume":"1","author":"Flatt JW","year":"2014","journal-title":"Thesis (Doctorate), Case Western Reserve University"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.20.11006-11015.2003"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.08.023"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.06.019"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.02.013"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.6b00834"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204522"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.5.3.435"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048177"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2014.06.029"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2017.06.013"},{"key":"e_1_2_8_1_22_1","first-page":"72","article-title":"Determination of adenoviruses subgenus from faecal samples obtained from children from Goi\u00e2nia\u2010Goi\u00e1s, Brazil","volume":"6","author":"Borges AMT","year":"2001","journal-title":"12th National Meeting of Virology and 4th Mercosul Meeting of Virology, 2001, Caldas Novas\u2010Goi\u00e1s. Virus Reviews & Research. Belo Horizonte\u2010MG:Revista da Sociedade Brasileira de Virologia"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-010-0748-3"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2008.03.007"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201100238"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-12-82"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac048455k"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800562"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.051151"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M114.045849"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-4-r58"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.07.006"},{"key":"e_1_2_8_1_34_1","unstructured":"FanYStudying the Effect of Human Adenovirus 5 and 36 on Host Cell Metabolism. Thesis (Doctorate) Princeton University 2015:157."},{"key":"e_1_2_8_1_35_1","first-page":"1","article-title":"Cardoso Cardoso DDDP. Proteomic analysis of A\u2010549 cells infected with human adenovirus 40 by LC\u2010MS","volume":"55","author":"Guissoni ACP","year":"2018","journal-title":"Virus Genes"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.19.9962-9971.2002"},{"key":"e_1_2_8_1_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.18.10122-10132.2004"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(80)90177-4"},{"key":"e_1_2_8_1_39_1","first-page":"430","article-title":"Regulated tissue\u2010specific expression of antagonistic pre\u2010mRNA splicing factors","volume":"4","author":"Hanamura A","year":"1998","journal-title":"RNA"},{"key":"e_1_2_8_1_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M900338-MCP200"},{"key":"e_1_2_8_1_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.3.4.662"},{"key":"e_1_2_8_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(79)90081-0"},{"key":"e_1_2_8_1_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.14.7707-7716.2004"},{"key":"e_1_2_8_1_44_1","doi-asserted-by":"publisher","DOI":"10.1185\/030079902125000732"},{"key":"e_1_2_8_1_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/35025102"},{"key":"e_1_2_8_1_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-90-481-2976-8_3"},{"key":"e_1_2_8_1_47_1","doi-asserted-by":"publisher","DOI":"10.2307\/3579375"},{"key":"e_1_2_8_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.02.038"},{"key":"e_1_2_8_1_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2014.03.009"},{"key":"e_1_2_8_1_50_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00712.2009"},{"key":"e_1_2_8_1_51_1","doi-asserted-by":"publisher","DOI":"10.2337\/db07-1313"},{"key":"e_1_2_8_1_52_1","first-page":"425","article-title":"Adenovirus E1A proteins and transformation","volume":"5","author":"Bayley ST","year":"1994","journal-title":"Int J Oncol"},{"key":"e_1_2_8_1_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00104-08"},{"key":"e_1_2_8_1_54_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.7.4.546"},{"key":"e_1_2_8_1_55_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.7.4.535"},{"key":"e_1_2_8_1_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208836"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.25439","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.25439","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.25439","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.25439","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T22:29:09Z","timestamp":1694384949000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.25439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,11]]},"references-count":55,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1002\/jmv.25439"],"URL":"https:\/\/doi.org\/10.1002\/jmv.25439","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,11]]},"assertion":[{"value":"2018-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}