{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:08:24Z","timestamp":1790615304628,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T00:00:00Z","timestamp":1552348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Centre Pasteur Cameroon"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rubella is an acute and contagious viral infection whose gravidity resides in infection during pregnancy, which can result in miscarriage, fetal death, stillbirth, or infants with congenital malformations. This study aimed to describe the genome of rubella viruses (RUBVs) circulating in Cameroon. Throat swabs were collected from health districts as part of the measles surveillance program from 2010 to 2016 and sent to the Centre Pasteur of Cameroon. Samples were amplified by genotyping reverse transcription polymerase chain reaction (RT\u2010PCR) in the search of two overlapping fragments of the gene that encodes the E1 envelope glycoprotein of RUBV. PCR products were sequenced and phylogenetic analysis was performed with MEGA 6 software. Overall, 9 of 43 samples (20.93%) were successfully amplified and sequenced but only eight sequences could be exploited for phylogenetic analysis with respect to the required fragment length of 739 nucleotides. Analysis of viral sequences from Cameroon with other epidemiologically relevant sequences from around the world showed that all RUBVs belonged to lineage L1 of genotype 1G. Cameroon sequences clustered with viruses from West Africa including Nigeria, Ivory Coast, and Ghana with a percentage similarity of 95.4% to 99.2%. This study will enable an update on the molecular epidemiology of RUBV in Cameroon and help in monitoring circulating RUBV for a better implementation of elimination strategies.<\/jats:p>","DOI":"10.1002\/jmv.25445","type":"journal-article","created":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T17:41:05Z","timestamp":1551462065000},"page":"928-934","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["First report of the genomic characterization of rubella viruses circulating in Cameroon"],"prefix":"10.1002","volume":"91","author":[{"given":"Franck\u2010Martin","family":"Obam Mekanda","sequence":"first","affiliation":[{"name":"Centre Pasteur of Cameroon, WHO National Reference Measles Laboratory Yaound\u00e9 Cameroon"},{"name":"University of Ngaound\u00e9r\u00e9, Faculty of Science Ngaound\u00e9r\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chavely Gwladys","family":"Monamele","sequence":"additional","affiliation":[{"name":"Centre Pasteur of Cameroon, WHO National Reference Measles Laboratory Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9dy Brice","family":"Simo Nemg","sequence":"additional","affiliation":[{"name":"Centre Pasteur of Cameroon, WHO National Reference Measles Laboratory Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francine Berlange","family":"Sado Yousseu","sequence":"additional","affiliation":[{"name":"Centre Pasteur of Cameroon, WHO National Reference Measles Laboratory Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieudonn\u00e9","family":"Ndjonka","sequence":"additional","affiliation":[{"name":"University of Ngaound\u00e9r\u00e9, Faculty of Science Ngaound\u00e9r\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anfumbom Kitu Womeyi","family":"Kfutwah","sequence":"additional","affiliation":[{"name":"World Health Organization, IST Libreville Gabon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Abernathy","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Division of Viral Diseases Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9275-0874","authenticated-orcid":false,"given":"Maurice","family":"Demanou","sequence":"additional","affiliation":[{"name":"Centre Pasteur of Cameroon, WHO National Reference Measles Laboratory Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,12]]},"reference":[{"key":"e_1_2_7_1_2_1","unstructured":"World Health Organization. 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