{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T19:03:16Z","timestamp":1769194996299,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T00:00:00Z","timestamp":1552867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Parvovirus B19 (PVB19) is transmitted via transfusion of blood and blood products. PVB19 is resistant to viral inactivation methods, which poses a threat to blood safety. We investigated the prevalence of PVB19 antibodies and DNA in healthy blood donors from the South African National Blood Bank Service to evaluate the necessity of PVB19 DNA testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Method<\/jats:title><jats:p>A retrospective analysis of 1500 residual plasma specimens from healthy blood donors from the SANBS repository were screened in mini\u2010pools of 20 for PVB19 DNA using a quantitative polymerase chain reaction (PCR). Positive pools were resolved by individual viral load testing and screened for PVB19 immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies to correlate viral loads with serological status. PVB19 IgG prevalence was determined by testing 90 randomly selected specimens from the 1500 plasma specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The prevalence of PVB19 IgG, IgM and IgG, and DNA was 62.2%, 0.06%, and 0.9%, respectively. Fourteen of the 1500 blood donor specimens received, had detectable PVB19 viral loads. Nine of the fourteen donors with detectable viral loads were PVB19 IgG seropositive. The PVB19 viral loads ranged from 1.81 to 5.32\u2009log IU\/mL. Four of the fourteen viraemic donors had a viraemia &gt;10\n<jats:sup>4<\/jats:sup> IU\/mL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We have demonstrated a low prevalence of PVB19 DNA in SANBS blood donors. The predominance of low\u2010level viraemia and the presence of PVB19 antibodies, suggests that the risk of transfusion transmission of PVB19 among SANBS donors may be relatively low.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.25450","type":"journal-article","created":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T18:48:36Z","timestamp":1551898116000},"page":"1217-1223","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Parvovirus B19 in South African blood donors"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5211-7241","authenticated-orcid":false,"given":"Kerri Lee","family":"Francois","sequence":"first","affiliation":[{"name":"Department of Virology National Health Laboratory Service and University of KwaZulu\u2010Natal Durban KwaZulu\u2010Natal South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raveen","family":"Parboosing","sequence":"additional","affiliation":[{"name":"Department of Virology National Health Laboratory Service and University of KwaZulu\u2010Natal Durban KwaZulu\u2010Natal South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pravi","family":"Moodley","sequence":"additional","affiliation":[{"name":"Department of Virology National Health Laboratory Service and University of KwaZulu\u2010Natal Durban KwaZulu\u2010Natal South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,18]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tracli.2015.11.006"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00004"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2007.00765.x"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00684-10"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-1053"},{"key":"e_1_2_8_1_7_1","unstructured":"US Department of Health and Human Services FaDA Center for Biologics Evaluation and Research. Nucleic Acid Testing (NAT) to Reduce the Possible Risk of Human Parvovirus B19 Transmission by Plasma\u2010Derived Products. Available from:http:\/\/www.fda.gov\/downloads\/BiologicsBloodVaccines\/GuidanceComplianceRegulatoryInformation\/Guidances\/Blood\/ucm078510.pdf.2009."},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-225706"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12295"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12162"},{"issue":"2","key":"e_1_2_8_1_11_1","first-page":"184","article-title":"Human parvovirus B19 and blood product safety: a tale of twenty years of improvements","volume":"13","author":"Marano G","year":"2015","journal-title":"Blood Transfus"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000357650"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25195"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01341.x"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2010.01423.x"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01443.x"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.3343\/kjlm.2010.30.1.58"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.22.12169-12178.2004"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268804002730"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-016-0611-6"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03067.x"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2003.00299.x"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05485-0"},{"key":"e_1_2_8_1_24_1","volume-title":"Screening donated blood for transfusion\u2010transmissible infections: recommendations","author":"Organization WH","year":"2010"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00074-09"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1940"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-015-0396-z"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.03047.x"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01704.x"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02613-13"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1086\/505226"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mjafi.2012.11.009"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12288-015-0607-1"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra030840"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2016.09.004"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.25450","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.25450","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.25450","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.25450","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T22:28:04Z","timestamp":1694384884000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.25450"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,18]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1002\/jmv.25450"],"URL":"https:\/\/doi.org\/10.1002\/jmv.25450","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,18]]},"assertion":[{"value":"2018-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}