{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T07:27:29Z","timestamp":1768030049468,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T00:00:00Z","timestamp":1554249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Human adenovirus (HAdV) infection can cause substantial morbidity in kidney transplant (KT) recipients. Cell\u2010mediated immunity plays an important role in controlling HAdV infection after KT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We prospectively (January 2015 to June 2018) investigated the absolute lymphocyte count (ALC) and interferon\u2010\u03b3\u2010producing CD4<jats:sup>+<\/jats:sup> and CD8\n<jats:sup>+<\/jats:sup> T cells at diagnosis and at viral clearance by an intracellular cytokine assay after stimulating with HAdV whole lysate, hexon, and penton proteins. HAdV infection was defined as the presence of HAdV DNA load in plasma or clinical specimens measured by the polymerase chain reaction assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eighteen adult KT recipients were diagnosed with HAdV infection at a median of 16 months (interquartile range [IQR], 2\u201039) after KT. The majority (94%) had HAdV\u2010associated hemorrhagic cystitis. The median ALC at viral clearance was significantly higher compared with diagnosis (2257 cells\/mm<jats:sup>3<\/jats:sup> [IQR, 1544\u20103078] vs 1001 cells\/mm<jats:sup>3<\/jats:sup> [IQR, 641\u20101385]; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Eleven patients underwent measurement of the HAdV\u2010specific T\u2010cell response. The median numbers of CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells at viral clearance were significantly higher compared with diagnosis (448 cells\/mm<jats:sup>3<\/jats:sup> [IQR, 248\u2010651] vs 215 cells\/mm<jats:sup>3<\/jats:sup> [IQR, 159\u2010272], <jats:italic>P<\/jats:italic>\u2009=\u20090.02; and 623 cells\/mm<jats:sup>3<\/jats:sup> [IQR, 242\u2010772] vs 235 cells\/mm<jats:sup>3<\/jats:sup> [IQR, 129\u2010266], <jats:italic>P<\/jats:italic>\u2009<jats:italic>&lt;<\/jats:italic>\u20090.01), respectively. The median percentages of penton\u2010specific CD4<jats:sup>+<\/jats:sup> and hexon\u2010specific CD8<jats:sup>+<\/jats:sup> T cells at viral clearance were significantly higher compared with diagnosis (0.012% vs 0%, <jats:italic>P<\/jats:italic>\u2009=\u20090.03%; and 0.136% vs 0.016%, <jats:italic>P<\/jats:italic>\u2009=\u20090.003, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest a trend of ALC and HAdV\u2010specific T\u2010cell immune restoration in KT recipients who achieve successful HAdV clearance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.25468","type":"journal-article","created":{"date-parts":[[2019,3,24]],"date-time":"2019-03-24T11:49:47Z","timestamp":1553428187000},"page":"1432-1439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Absolute lymphocyte count and human adenovirus\u2013specific T\u2010cell immune restoration of human adenovirus infection after kidney transplantation"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0930-8936","authenticated-orcid":false,"given":"Jackrapong","family":"Bruminhent","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine Faculty of Medicine Ramathibodi Hospital, Mahidol University Bangkok Thailand"},{"name":"Excellence Center of Organ Transplantation Faculty of Medicine Ramathibodi Hospital, Mahidol University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nopporn","family":"Apiwattanakul","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Pediatrics Faculty of Medicine Ramathibodi Hospital, Mahidol University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suradej","family":"Hongeng","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Pediatrics Faculty of Medicine Ramathibodi Hospital, Mahidol University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surasak","family":"Kantachuvesiri","sequence":"additional","affiliation":[{"name":"Excellence Center of Organ Transplantation Faculty of Medicine Ramathibodi Hospital, Mahidol University Bangkok Thailand"},{"name":"Division of Nephrology, Department of Medicine Faculty of Medicine Ramathibodi Hospital, Mahidol University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siriorn P.","family":"Watcharananan","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine Faculty of Medicine Ramathibodi Hospital, Mahidol University Bangkok 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