{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:48:41Z","timestamp":1791946121922,"version":"4.3.4"},"reference-count":12,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,26]],"date-time":"2019-05-26T00:00:00Z","timestamp":1558828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007586","name":"Aix-Marseille Universit\u00e9","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1115490"],"award-info":[{"award-number":["OPP1115490"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human respiratory syncytial virus (HRSV) is one of the most important causes of acute respiratory infections (ARI) in young children. HRSV diagnosis is based on the detection of the virus in respiratory specimens. Nasopharyngeal swabbing is considered the preferred method of sampling, although there is limited evidence of the superiority of nasopharyngeal swabs (NPS) over the less invasive nasal (NS) and throat (TS) swabs for virus detection by real\u2010time reverse transcription quantitative polymerase chain reaction (RT\u2010qPCR). In the current study, we compared the three swabbing methods for the detection of HRSV by RT\u2010qPCR in children hospitalized with ARI at Mahosot Hospital, Vientiane, Laos. In 2014, NS, NPS, and TS were collected from 288 children. All three samples were tested for HRSV by RT\u2010qPCR; 141 patients were found positive for at least one sample. Almost perfect agreements (<jats:italic>\u03ba<\/jats:italic>\u2009&gt;\u20090.8) between the swabs, compared two by two, were observed. Detection rates for the three swabs (between 93% and 95%) were not significantly different, regardless of the clinical presentation. Our findings suggest that the uncomfortable and technically more demanding NPS method is not mandatory for HRSV detection by RT\u2010qPCR.<\/jats:p>","DOI":"10.1002\/jmv.25496","type":"journal-article","created":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T10:33:35Z","timestamp":1556879615000},"page":"1602-1607","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Nasal or throat sampling is adequate for the detection of the human respiratory syncytial virus in children with acute respiratory infections"],"prefix":"10.1002","volume":"91","author":[{"given":"Van Hoan","family":"Nguyen","sequence":"first","affiliation":[{"name":"Unit\u00e9 des Virus \u00c9mergents (UVE: Aix\u2010Marseille Univ \u2010 IRD 190 \u2010 Inserm 1207 \u2010 IHU M\u00e9diterran\u00e9e Infection) Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona M","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Paediatrics The University of Melbourne Melbourne Australia"},{"name":"Pneumococcal Research Group, Murdoch Children's Research Institute The Royal Children's Hospital Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David AB","family":"Dance","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory Lao\u2010Oxford\u2010Mahosot Hospital\u2010Wellcome Trust Research Unit (LOMWRU) Vientiane Capital Lao PDR"},{"name":"Nuffield Department of Clinical Medicine, Centre for Tropical Medicine and Global Health University of Oxford Oxford United Kingdom"},{"name":"Faculty of Infectious and Tropical Diseases London School of Hygiene and Tropical Medicine London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keoudomphone","family":"Vilivong","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory Lao\u2010Oxford\u2010Mahosot Hospital\u2010Wellcome Trust Research Unit (LOMWRU) Vientiane Capital Lao PDR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souphatsone","family":"Phommachan","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory Lao\u2010Oxford\u2010Mahosot Hospital\u2010Wellcome Trust Research Unit (LOMWRU) Vientiane Capital Lao PDR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanthaphone","family":"Syladeth","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory Lao\u2010Oxford\u2010Mahosot Hospital\u2010Wellcome Trust Research Unit (LOMWRU) Vientiane Capital Lao PDR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Lai","sequence":"additional","affiliation":[{"name":"Pneumococcal Research Group, Murdoch Children's Research Institute The Royal Children's Hospital Melbourne 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