{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T01:03:32Z","timestamp":1783040612573,"version":"3.54.6"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T00:00:00Z","timestamp":1566345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous hepatitis E virus (HEV) seroprevalence studies in Turkey have shown high variabilities,\u00a0leading to conflicting results. We aimed to re\u2010evaluate HEV seroprevalence among blood donors in Turkey using the Wantai (Beijing, China) and the Dia.Pro (Milan, Italy) total anti\u2010HEV antibody (Ab) enzyme\u2010linked immunosorbent assay (ELISA) kits and compare their performances and to investigate the presence of HEV RNA in blood donors. Serum total anti\u2010HEV antibodies were determined in a total of 2011 volunteer blood donor samples collected from different regions of Turkey (807 from Ankara, 243 from Kayseri, 284 from \u0130zmir, 200 from Malatya, 200 from Kahramanmara\u015f, and 277 from Van). HEV RNA was evaluated by a real\u2010time polymerase chain reaction in a total of 272 anti\u2010HEV seropositive samples. The country\u2010wide HEV seroprevalence was calculated as 11.5% (Dia.Pro) and 12.2% (Wantai) with seropositivity rates of 12.0%\u201012.5% in Ankara, 7.4%\u20108.2% in Kayseri, 14.5%\u201015.5% in Malatya, 8.1%\u20108.8% in \u0130zmir, 15.0%\u201016.0% in Kahramanmara\u015f, and 12.6%\u201013.4% in Van by Dia.Pro and Wantai kits, respectively. The lowest detectable Ab concentrations were 0.16 and 0.14\u2009units\/mL WHO, for the Dia.Pro and the Wantai assays, respectively, showing no significant difference between assays. HEV RNA was not detected in any of the anti\u2010HEV seropositive samples. Compared with previous studies, HEV was shown to have a higher overall seroprevalence in Turkey. Despite its limitation, the current study represents the most comprehensive HEV seroprevalence study in Turkey performed with two different commercial ELISA assays with high sensitivities so far. Further investigation is required to determine HEV genotypes in Turkey.<\/jats:p>","DOI":"10.1002\/jmv.25567","type":"journal-article","created":{"date-parts":[[2019,8,12]],"date-time":"2019-08-12T09:15:31Z","timestamp":1565601331000},"page":"2174-2181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["HEV seroprevalence in blood donors in Turkey by two commercial total anti\u2010HEV Ab ELISA kits"],"prefix":"10.1002","volume":"91","author":[{"given":"Osman","family":"Ya\u015far","sequence":"first","affiliation":[{"name":"Institute of Hepatology Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ersin","family":"Karatayli","sequence":"additional","affiliation":[{"name":"Institute of Hepatology Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcniz","family":"Cengiz","sequence":"additional","affiliation":[{"name":"Institute of Hepatology Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehtap","family":"K\u0131z\u0131lp\u0131nar","sequence":"additional","affiliation":[{"name":"Institute of Hepatology Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esra","family":"Yurdcu","sequence":"additional","affiliation":[{"name":"Institute of Hepatology Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabia","family":"Albayrak","sequence":"additional","affiliation":[{"name":"Department of Biometry and Genetics, Faculty of Agriculture Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aysel","family":"G\u00fcven","sequence":"additional","affiliation":[{"name":"Vocational School of Health Ba\u015fkent University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6nder","family":"Arslan","sequence":"additional","affiliation":[{"name":"Department of Hematology, School of Medicine Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ceren","family":"Karahan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Clinical Microbiology, School of Medicine Ankara University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bar\u0131\u015f","family":"Otlu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine In\u00f6n\u00fc University  Malatya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcseyin","family":"G\u00fcd\u00fcc\u00fco\u011flu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Van Y\u00fcz\u00fcnc\u00fc Y\u0131l University  Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma","family":"G\u00f6kahmeto\u011flu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elife","family":"Berk","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zlem","family":"Kiri\u015fci","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology Necip Faz\u0131l City Hospital  Kahramanmara\u015f Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00fc\u00e7han","family":"Sert\u00f6z","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Ege University  \u0130zmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cihan","family":"Yurdaydin","sequence":"additional","affiliation":[{"name":"Institute of Hepatology Ankara University  Ankara Turkey"},{"name":"Department of Gastroenterology and Hepatology Koc University Medical School  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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