{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:32:34Z","timestamp":1762522354012,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T00:00:00Z","timestamp":1567900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human papillomavirus (HPV) detection is used for screening of cervical cancer and genotype\u2010specific persistence has shown to be mandatory for dysplasia development. Aim of this study was to evaluate the clinical performance of HPV DNA Array for cervical intraepithelial neoplasia\u00a02+ (CIN2+) \u2009lesion detection. HPV DNA Array is a polymerase chain reaction\u2010based assay that targets E1 sequences of 29 HPV types (6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 44, 45, 51, 52, 53, 54, 56, 58, 59, 66, 67, 68, 69, 70, 73, 82, 85, and 97). The clinical evaluation was performed against the reference assay, BS\u2010GP5+\/6+\u2009multiplex genotyping (MPG)\u2010Luminex, with 600 cervical smear samples of a referral population. HPV DNA Array detected CIN2+\u2009 lesions with a sensitivity of 90.2%, identical to that of MPG\u2010Luminex. Detection of CIN3+\u2009 lesions was with a sensitivity of 90.3%, as compared with 88.7% of MPG\u2010Luminex. It demonstrated very good agreement for HPV detection, irrespective of type, of 91.5% (<jats:italic>\u03ba<\/jats:italic>\u2009\u2009=\u2009\u20090.832). HPV DNA Array is a simple and robust assay, with a short protocol of 4\u2009hours hands\u2010on time and automated readout by ELISpot AiDot software. It permits testing of up to 96 samples in one run and may be considered for use in organized screening programs and low resource settings.<\/jats:p>","DOI":"10.1002\/jmv.25583","type":"journal-article","created":{"date-parts":[[2019,8,31]],"date-time":"2019-08-31T09:04:31Z","timestamp":1567242271000},"page":"113-118","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Clinical performance of the HPV DNA Array genotyping assay in detection of CIN2+ lesions with BS GP5+\/6+\u00a0MPG Luminex tested cervical samples"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2399-3298","authenticated-orcid":false,"given":"Aleksandra","family":"Pesic","sequence":"first","affiliation":[{"name":"Gynaecology Clinic, Charit\u00e9 Universit\u00e4tsmedizin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t Berlin and Berlin Institute of Health Berlin 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Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7732-3009","authenticated-orcid":false,"given":"Andreas M.","family":"Kaufmann","sequence":"additional","affiliation":[{"name":"Gynaecology Clinic, Charit\u00e9 Universit\u00e4tsmedizin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t Berlin and Berlin Institute of Health Berlin 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