{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T04:30:58Z","timestamp":1782016258839,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:00:00Z","timestamp":1575849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In 2017, Tamil Nadu, a southern state, had the second highest number of dengue cases from India. In the present study, the serotype\u2010specific differences in the clinical manifestations and laboratory parameters among hospitalized children with dengue were investigated and molecular characterization of the circulating dengue virus (DENV) serotypes during 2017 in Tamil Nadu was performed. Eighty children with dengue\u2010like symptoms consecutively admitted to a tertiary care hospital and positive for DENV NS1 antigen were investigated for DENV serotype utilizing a real\u2010time reverse transcriptase based polymerase chain reaction assay. Complete envelope (E) gene sequencing of the DENV strains was performed. Seventy samples were positive for serotyping (25 DENV\u20101, 17 DENV\u20102, six DENV\u20103, and 22 DENV\u20104). DENV\u20104 infections were associated with elevated levels of liver enzymes; Alanine aminotransferase (<jats:italic>P<\/jats:italic>\u2009=\u2009.021) and aspartate\u00a0aminotransferase (<jats:italic>P<\/jats:italic>\u2009=\u2009.001). However, none of the serotype was associated with any specific clinical features and severe dengue. Asian and American\/African genotypes of DENV\u20101 were cocirculating. The circulating genotype was cosmopolitan for DENV\u20102 with multiple lineages, genotype III for DENV\u20103 and genotype I for DENV\u20104. Unique mutations were present in the 2017 DENV\u20104 isolates. The present study suggests the association of DENV\u20104 with elevated liver enzymes in children hospitalized for dengue. Further, the study reports the genetic diversity of DENV circulating in Tamil Nadu during 2017. The study calls for continuous monitoring of the circulating serotypes and genotypes at regional level in India which might result in a region wise database useful in predicting future outbreaks.<\/jats:p>","DOI":"10.1002\/jmv.25639","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T10:36:06Z","timestamp":1574764566000},"page":"1013-1022","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Serotype\u2010specific differences in the laboratory parameters among hospitalized children with dengue and genetic diversity of dengue viruses circulating in Tamil Nadu, India during 2017"],"prefix":"10.1002","volume":"92","author":[{"given":"N. 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