{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:41:22Z","timestamp":1790286082380,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T00:00:00Z","timestamp":1576627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","award":["UMCoE Top 100 Research Grant: UM.00000188\/HGA.GV"],"award-info":[{"award-number":["UMCoE Top 100 Research Grant: UM.00000188\/HGA.GV"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","award":["University Research Grant 2018: RU008\u20102018"],"award-info":[{"award-number":["University Research Grant 2018: RU008\u20102018"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV) are mosquito\u2010borne flavivirus of medical importance in tropical countries such as Malaysia. However, much remains unknown regarding their prevalence among the underserved indigenous people (Orang Asli) living in communities in the forest fringe areas of Peninsular Malaysia. Information on the prevalence of diseases is necessary to elevate the effectiveness of disease control and preventive measures. This study aimed to determine the seroprevalence of the three major flaviviruses among the Orang Asli and investigate the association between demographic factors and seropositivities. Sampling activities were conducted in the Orang Asli villages to obtain serum samples and demographic data from consenting volunteers. The presence of DENV, JEV, and ZIKV immunoglobulin G (IgG) antibodies in the sera were examined using commercial enzyme\u2010linked immunosorbent assay kits. A focus reduction neutralization assay was performed to measure virus\u2010specific neutralizing antibodies. A total of 872 serum samples were obtained from the Orang Asli volunteers. Serological assay results revealed that DENV IgG, JEV IgG, and ZIKV IgG seropositivities among the Orang Asli were at 4.9%, 48.4%, and 13.2%, respectively. Neutralizing antibodies (FRNT50\u2009\u2265\u20091:40) against JEV and ZIKV were found in 86.7% and 100.0%, respectively, out of the samples tested. Positive serology to all three viruses corresponded significantly to the age of the volunteers with increasing seropositivity in older volunteers. Findings from the study suggest that Orang Asli are at significant risk of contracting JEV and ZIKV infections despite the lack of active transmission of the viruses in the country.<\/jats:p>","DOI":"10.1002\/jmv.25649","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T05:51:23Z","timestamp":1575870683000},"page":"956-962","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Serological evidence of DENV, JEV, and ZIKV among the indigenous people (Orang Asli) of Peninsular Malaysia"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2883-3031","authenticated-orcid":false,"given":"Chee\u2010Sieng","family":"Khor","sequence":"first","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurul\u2010Farhana","family":"Mohd\u2010Rahim","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"},{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habibi","family":"Hassan","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"},{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim\u2010Kee","family":"Tan","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurhafiza","family":"Zainal","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"},{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boon\u2010Teong","family":"Teoh","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sing\u2010Sin","family":"Sam","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010Jing","family":"Khoo","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Yen","family":"Lee","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne Ai\u2010Lian","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sazaly","family":"Abubakar","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research &amp; Education Centre University of Malaya  Kuala Lumpur Malaysia"},{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,18]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Ministry of Rural and Regional Development Malaysia. 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