{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T23:34:42Z","timestamp":1775086482291,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T00:00:00Z","timestamp":1580688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>West Nile virus (WNV) is a neurotropic arbovirus that can also be transmitted through blood transfusion. Even though its geographic distribution has been expanding, there has not yet been any epidemiological data on WNV in northern Cyprus. The aim of our study is to fill this gap by using donated blood samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Samples collected from the main government hospital blood bank in Nicosia were analyzed by anti\u2010WNV enzyme\u2010linked immunosorbent assay (ELISA) (immunoglobulin M [IgM] and immunoglobulin G [IgG]). Seropositive samples were subjected to plaque reduction neutralization test (PRNT) for confirmation and analyzed by ELISA IgG avidity test and reverse transcription real\u2010time polymerase chain reaction (rRT\u2010PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 760 sera samples, 2 (0.3%) were IgM+ and 31 (4.1%) were IgG+. Neutralization activity was detected in none (0.0%) of the IgM+ and 26 (83.9%) of IgG+ donor specimens. ELISA IgG avidity test reported high avidity in 21 (67.7%) and low avidity in one (3.2%) IgG+ sample. PRNT\u2010confirmed anti\u2010WNV IgG+ samples exhibited only borderline (19.2%) or high avidity (80.8%) values. rRT\u2010PCR results were negative for both IgM+ and IgG+ samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Anti\u2010WNV antibodies were detected in northern Cyprus among blood donors. The establishment of preventive measures and evaluation of the geographic extent of the WNV in northern Cyprus are highly recommended.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.25669","type":"journal-article","created":{"date-parts":[[2020,1,11]],"date-time":"2020-01-11T08:33:48Z","timestamp":1578731628000},"page":"1035-1039","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Serological screening of West Nile virus among blood donors in northern Cyprus"],"prefix":"10.1002","volume":"92","author":[{"given":"Nagat","family":"Balaman","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine Near East University Nicosia Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9945-478X","authenticated-orcid":false,"given":"Umut","family":"Gazi","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine Near East University Nicosia Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Turgut","family":"Imir","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine Near East University Nicosia Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamer","family":"Sanlidag","sequence":"additional","affiliation":[{"name":"Research Centre of Experimental Health Sciences Near East University Nicosia Cyprus"},{"name":"Department of Medical Microbiology, Faculty of Medicine Celal Bayar University Manisa Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emrah","family":"Ruh","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine Near East University Nicosia Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ozgur","family":"Tosun","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Faculty of Medicine Near East University Nicosia Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aykut","family":"Ozkul","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Veterinary Medicine Ankara University Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aysegul","family":"Taylan\u2010Ozkan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine Near East University Nicosia Cyprus"},{"name":"Department of Medical Microbiology, Faculty of Medicine Hitit University Corum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/376230"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000446219"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00045-12"},{"key":"e_1_2_6_5_1","unstructured":"Centers for Disease Control and Prevention.West Nile virus (WNV) fact sheet.2016.https:\/\/www.cdc.gov\/westnile\/resources\/pdfs\/wnvFactsheet_508.pdf"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200108000-00016"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/23744235.2016.1164890"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02421"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2017.02.003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003028"},{"key":"e_1_2_6_11_1","unstructured":"Three cases of West Nile in the north.Cyprus Mail.https:\/\/cyprus\u2010mail.com\/2019\/07\/25\/three\u2010cases\u2010of\u2010west\u2010nile\u2010in\u2010the\u2010north\/. 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